Abstract
Adsorption and desorption of L-tryptophan (L-trp) on strong acid cation exchange resin were investigated in a fixed-bed column. L-trp was effectively adsorbed onto the resin HZ-001, with the loading capacity and breakthrough time determined. Four kinetic models, including Adams-Bohard, Wolborska, Thomas, and Yoon-Nelson models, were adopted to determine the kinetic parameters of adsorption and to predict the breakthrough curves. The results showedthat the models used described the breakthrough well. Desorption of L-trp from the column bed was performed usingaqueous ammonia as the eluant. Optimum procedure was obtained with 2.0 M aqueous ammonia at the elution velocityof 6 BV/h. Five cycles of adsorption-elution-regeneration were conducted to evaluate the column reutilization.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Z. N. Xu, Y. Chen, W. H. Shen and P. L. Cen, Korean J. Chem. Eng., 23, 108 (2006).
Y. Chen, H. B. Qu, Z. N. Xu and Y. Y. Cheng, Korean J. Chem. Eng., 23, 991 (2006).
D. Banerjee, M. A. Rao and S. K. Samanta, Solvent. Extr. Ion. Exc., 26, 687 (2008).
S. Melis, J. Markos, G. Cao and M. Morbidelli, Ind. Eng. Chem. Res., 35, 1912 (1996).
H. Nagai and G. Carta, Sep. Sci. Technol., 39, 3691 (2004).
S. Rengaraj, J.-W. Yeon, Y. Kim, Y. Jung, Y.-K. Ha and W.-H. Kim, J. Hazard. Mater., 143, 469 (2007).
M. H. L. Ribeiro, D. M. F. Prazeres, J. M. S. Cabral and M. M. R. d. F., Bioprocess Eng., 12, 95 (1995).
T. E. Köse and N. Öztürk, J. Hazard. Mater., 152, 744 (2008).
F. Alguacil, M. Alonso and L. Lozano, Chemosphere, 57, 789 (2004).
M. J. Ahmed, A. H. A. K. Mohammed and A. A. H. Kadhum, Korean J. Chem. Eng., 27, 752 (2010).
V. C. Srivastava, B. Prasad, I. M. Mishra, I. D. Mall and M. M. Swamy, Ind. Eng. Chem. Res., 47, 1603 (2008).
R. Han, D. Ding, Y. Xu, W. Zou, Y. Wang, Y. Li and L. Zou, Biore-source Technol., 99, 2938 (2008).
W. Luo, H. Chen, L. Fan, J. Huang, L. Huang, Z. Xu and P. Cen, Korean J. Chem. Eng. DOI:10.2478/s11814-010-0490-2.
J. R. Rao and T. Viraraghavan, Bioresource Technol., 85, 165 (2002).
Z. Aksu, F. Gönen and Z. Demircan, Process. Biochem., 38, 175 (2002).
A. C. Texier, Y. Andres, C. Faur-Brasquet and P. LeCloirec, Chemo-phere, 47, 333 (2002).
Z. Aksu and F. Gönen, Process. Biochem., 39, 599 (2004).
D. O. Cooney, Chem. Eng. Commun., 110, 217 (1991).
P. Malakul, D. Srinivasan and H. Y. Wang, Ind. Eng. Chem. Res., 37, 4296 (1998).
S. Lin, Chem. Eng. J., 92, 193 (2003).
S. H. Lin and C. S. Wang, J. Hazard. Mater., B90, 205 (2002).
V. Sarin, T. Singh and K. Pant, Bioresource Technol., 97, 1986 (2006).
X. Liao, M. Zhang and B. Shi, Ind. Eng. Chem. Res., 43, 2222 (2004).
Y. H. Yoon and J. H. Nelson, Am. Ind. Hyg. Assoc. J., 45, 509 (1984).
W. T. Tsai, C. Y. Chang, C. Y. Hoand L. Y. Chen, J. Hazard. Mater., 69, 53 (1999).
P. Ye, Z. Luan, K. Li, L. Yu and J. Zhang, Carbon, 47, 1799 (2009).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Luo, W., Wei, P., Chen, H. et al. Kinetics and optimization of L-tryptophan separation with ion-exchange chromatography. Korean J. Chem. Eng. 28, 1280–1285 (2011). https://doi.org/10.1007/s11814-010-0489-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-010-0489-8