Abstract
This paper describes a fed-batch fermentation protocol about production of selenium-enriched yeast. Saccharomyces cerevisiae GS2 was selected because of its high tolerance to selenium. The strain GS2 was tested and 122±0.5 g·l −1 dry cell weight was obtained after 30 h cultivation through feed back control of feed rate of glucose according to the concentration of ethanol and dissolved oxygen. Furthermore, the optimal pattern of Na2SeO3 addition was 9 mg Na2SeO3 against 1 g DCW at late exponential phase. With the combination of glucose feeding and Na2SeO3 addition, the final dry cell biomass reached 102±0.4 g·l −1 and a Se uptake level of 2,020±13 mg·Kg−1 was achieved in a 5 l fermentor after 38 h cultivation.
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References
C. Chen, P. Zhang, X. Hou and Z. Chai, Biochim. Biophys. Acta, 1427, 205 (1999).
L. C. Clark, G. F. Combs, B.W. Turnbull, E. H. Slate, D.K. Chalker, J. Chow, L. S. Davis, R.A. Glover, G. F. Graham, E.G. Gross, A. Krongrad, J. L. Lesher, H.K. Park, B. B. Sanders, C. L. Smith and J. R. Taylor, J. Am. Med. Assoc., 276, 1957 (1996).
J.W. Finley, C. Ip, D. J. Lisk, C.D. Davis, K. J. Hintze and P.D. Whanger, J. Agric. Food Chem., 49, 2679 (2001).
E.A. Klein, S. M. Lippman, I.M. Thompson, P. J. Goodman, D. Albanes, P. R. Taylor and C. Coltman, World J. Urol., 21, 21 (2003).
R. Bologna, F. Indacochea, G. Shor-Posner, E. Mantero-Atienza, M. Grazziutii, M. C. Sotomayor, M. Fletcher, C. Cabrejos, G. B. Scott and M. K. Baum, J. Nutr. Immunol., 3, 41 (1994).
L. Letavayová, V. Vlčková and J. Brozmanová, Toxicology, 227, 1 (2006).
A. Demirci, A. L. Pometto and D. J. Cox, J. Agric. Food Chem., 47, 2496 (1999).
A. Demirci and A. L. Pometto III, J. Agric. Food Chem., 47, 2491 (2000).
A. Suhajda, J. Hegoczki, B. Janzso, I. Pais and G. Vereczkey, J. Trace Elem. Med. Bio., 14, 43 (2000).
M.M. Gharieb and G.M. Gadd, Mycol. Res., 108, 1415 (2004).
Z. Wang, T. Tan and J. Song, Process Biochem., 42, 108 (2007).
F. Shang, Z. Wang and T. Tan, Appl. Microbiol. Biotechnol., 77, 1233 (2008).
H. Yin, Z. Chen, Z. Gu and H. Gao, LWT Food Sci. Technol., 42, 327 (2009).
T. Tan, M. Zhang and H. Gao, Enzyme Microb. Technol., 33, 366 (2003).
J. Chen, S. Zhang, J. Chu, Y. Zhuang, J. Luo and H. Bai, Biotechnol. Lett., 26, 109 (2004).
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Wang, Z., Zhang, L. & Tan, T. High cell density fermentation of Saccharomyces cerevisiae GS2 for selenium-enriched yeast production. Korean J. Chem. Eng. 27, 1836–1840 (2010). https://doi.org/10.1007/s11814-010-0300-x
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DOI: https://doi.org/10.1007/s11814-010-0300-x