Abstract
Lysozyme was extracted from aqueous solution into i-octane using reverse micelles in the presence of pressurized CO2. A squat vessel with two independent stirrers was used to measure the mass transfer of the lysozyme across a planar interface. Mass transfer coefficient, k L of the lysozyme from the aqueous to the organic phase was measured at selected ionic strengths, pH, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) surfactant concentrations, temperatures and pressurized CO2. The mass transfer rate of lysozyme was higher in high temperature (318 K) and pressure (20MPa). pH of 9 in aqueous phase showed highest mass transfer rate of lysozyme. The application of pressurized CO2 markedly increased the mass transfer rate of lysozyme comparing to conventional non-pressurized system.
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Jung, SM., Shin, UM., Uddin, M.S. et al. Transfer rate measurement of lysozyme by liquid-liquid extraction using reverse micelles with dense CO2 . Korean J. Chem. Eng. 27, 596–601 (2010). https://doi.org/10.1007/s11814-010-0063-4
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DOI: https://doi.org/10.1007/s11814-010-0063-4