Abstract
Immobilized-metal affinity chromatography (IMAC) media was prepared. Iminodiacetic acid (IDA) was optimally coupled to the oxirane-activated gel at pH 13.0 and 60 °C in 0.1–0.15 g of IDA per ml of 2M Na2CO3 for 5–7 hours. The amount of coupled IDA was 600–800 micromoles per gram of dried gel by determining zinc (II) ion with atomic absorption spectroscopy. Adsorption and desorption of protein sample to IDA-coupled media was made and the result is compatible to ones reported previously. The efficiency of column chromatography was discussed on partially purifying β-galactosidase from E. coli as the protein sample by zinc (II) ion chelate affinity column.
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Kim, JH., Hwang, W.S. & Kim, I.H. Simple preparation of immobilized-metal affinity chromatography media. Korean J. Chem. Eng. 26, 1693–1695 (2009). https://doi.org/10.1007/s11814-009-0276-6
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DOI: https://doi.org/10.1007/s11814-009-0276-6