Abstract
The saccharogenic liquid obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes (SFW) was carried out by the cultivation supernatant of Trichoderma inhamatum KSJ1. 5.6 g/L of BC was produced in a new modified 50 L bubble column bioreactor by Acetobacter xylinum KJ1. The productivity was similar to that of a modified 10 L bubble column bioreactor (5.8 g/L). When pure oxygen was supplied into the scaled-up culture conditions, 6.8 g/L (12% enhancement) of BC was produced, indicating a very useful method for BC mass production. The oxygen uptake rate (OUR) and q O2 (specific oxygen uptake rate) were 0.214 mg-DO/L·min and 0.257 mg-DO/g-cell·min, respectively. The physical properties, such as morphology, molecular weight, crystallinity, and tensile strength of BCs produced in static culture (A), 10 L (B) and 50 L (C) modified bubble column cultures were investigated. All BCs showed fibrils with highly networking structure. The number average molecular weight of BCs in A, B and C was 2,314,000, 1,878,000, and 1,765,000, respectively. All of the BCs had a form of cellulose I representing pure cellulose. The relative degree of crystallinity showed the range of 79.6–86.0%. Tensile strengths of BC sheet in A, B and C were 1.75, 1.21, and 1.19 kg/mm2, respectively. In conclusion, BC production by the modified bubble column culture mode of 50 L brought more favorable results in terms of the physical properties and its ease of scale-up.
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References
D. Klemm, D. Schumann, U. Udhard and S. Marsch, Prog. Polym. Sci., 26, 1561 (2001).
H. Shibazaki, S. Kuga, F. Onabe and M. Usuda, J. Appl. Polym. Sci., 50, 965 (1993).
S. Valla and J. Kjosbakken, J. Gen. Microbiol., 128, 1401 (1982).
M. Matsuoka, T. Tsuchida, K. Matushita, O. Adachi and F. Yoshinaga, Biosci. Biotechnol. Biochem., 60, 575 (1996).
T. Naritomi, T. Kouda, H. Yan and F. Yoshinaga, J. Ferment. Bioeng., 85, 89 (1998).
S. H. Moon, J. M. Park, H. Y. Chun and S. J. Kim, Biotechnol. Bioprocess Eng., 11, 26 (2006).
M. Mandel and D. Sternberg, J. Ferment. Tech., 54, 267 (1976).
H. Taguchi and A. E. Humphrey, J. Ferment. Tech., 44(12), 881 (1966).
W. J. Alexander and R. L. Michell, Anal. Chem., 21, 1497 (1949).
L. Segal, J. Creely, A. Martin and C. Conrad, Text. Res. J., 29, 786(1959).
Annual Book of ASTM Standards, section 8, Plastics, ed. by ASTM, Pennsylvania, Vol. 8 (1993).
T. Ishida, M. Mitarai, Y. Sugano and M. Shoda, Biotechnol. Bioeng., 83(4), 474 (2003).
Y. Chao, T. Ishida, Y. Sugano and M. Shoda, Biotechnol. Bioeng., 68(3), 345 (2000).
M. Shoda and Y. Sugano, Biotechnol. Bioprocess Eng., 10, 1 (2005).
Y. Nishi, M. Uryu, S. Yamanaka, K. Watanabe, N, Kitamura, M. Iguchi and S. Mitsuhashi, J. Mater. Sci., 25, 2997 (1990).
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Choi, C.N., Song, H.J., Kim, M.J. et al. Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor. Korean J. Chem. Eng. 26, 136–140 (2009). https://doi.org/10.1007/s11814-009-0021-1
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DOI: https://doi.org/10.1007/s11814-009-0021-1