Abstract
Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HHx)) was produced by Aeromonas sp. KC014 strain isolated from Taiwan in soil environment in the flask and fermentor cultures. The medium optimization, such as carbon source and nitrogen source, carbon-nitrogen ratio was conducted to obtain the optimum 3-hydroxyhexanoate content. The defined medium with dodecanoic acid as the carbon source and (NH4)2SO4 as the nitrogen source was obtained as the main medium. When cells grown in medium containing 30 g/L dodecanoic acid, 15 g/L sodium gluconate and 1 g/L soytone (C/N was 30/1) as final PHA concentration, the cell dry weight and HB content of 5.16 g/L, 14.0 g/L and 36.0%, respectively, were obtained. The maximum HHx/PHA content increased from 0.1% to 1.3% nearly 12-fold when the dissolved oxygen was decreased from 40% to 20%. P(3HB-co-3HHx) biosynthesis was triggered by the addition of limited nitrogen, phosphorus and magnesium to get a maximum HHx/PHA content of 14% in 95 hours.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Y.H. Wei, C.W. Lo, C. C. Chien, S. S. Kung, C. S. Liao, Y. M. Sun and H. S. Wu, Trends in Chem. Eng., 9, 27 (2005).
K. Sudesh, H. Abe and Y. Doi, Prog. Polym. Sci., 25, 1503 (2000).
H. S. Byun and H.Y. Lee, Korean J. Chem. Eng., 23, 1003 (2006).
B. C. Lee, Korean J. Chem. Eng., 20, 542 (2003).
K.H. Song, C.H. Lee, J. S. Lim and Y.W. Lee, Korean J. Chem. Eng., 19, 139 (2002).
T. Fukui and Y. Doi, RIKEN Review, 15, 25(1997).
G. Kobayashi, T. Shiotani, Y. Shima and Y. Doi, In: Doi Y, Fukuda K, editors, Biodegradable plastics and polymers, Amsterdam: Elsevier, 410 (1994).
Y. Z. Qiu, J. Han and G.Q. Chen, Appl. Microbiol. Biotechnol., 69, 537 (2006).
Y. Z. Qiu, J. Han, J. J. Guo and G.Q. Chen, Biotech. Let., 27, 1381 (2005).
Y. Z. Qiu, S. P. Quyang, Z.Y. Shen, Q. Wu and G.Q. Chen, Macromol. Biosci., 4, 255 (2004).
S. Quyang, J. Han, Y. Qiu, L. Qin, S. Chen, Q. Wu, M. L. Leski and G. Q. Chen, Macromol. Symp., 224, 21 (2005).
I. C. Jhuang, MS Thesis. Yuan Ze University, Taiwan (2005).
G.Q. Chen, G. Zhang, S. J. Park and S.Y. Lee, Appl. Microbiol. Biotechnol., 57, 50 (2001).
S.Y. Lee, J. Choi and H.H. Wong, Intern.l J. Bio. Macro., 25, 31 (1999).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Gong, YA., Wu, HS., Wei, YH. et al. Fermentation strategy for the production of poly(3-hydroxyhexanoate) by Aeromonas sp. KC014. Korean J. Chem. Eng. 25, 1422–1426 (2008). https://doi.org/10.1007/s11814-008-0233-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-008-0233-9