Abstract
The activity and stability of lipase from Candida antarctica were investigated in the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate using ionic liquids (ILs) as reaction media. Among ILs tested, the highest activity of lipase was observed in [Edmim][Tf2N]. In hydrophobic ILs such as [Edmim][Tf2N], [Emim][Tf2N] and [Pmim] [PF6], lipase could retain its activity after 5 times reuse, while the activity of lipase in hydrophilic ILs and organic solvents was drastically decreased. The activities of lipase in [Edmim][Tf2N], [Emim][Tf2N] and [Pmim][PF6] were also well maintained after 1 day incubation at 80 °C. The lipase suspended in [Edmim][Tf2N] could be successfully reused 6 times without loss of activity.
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Ha, S.H., Lee, S.H., Dang, D.T. et al. Enhanced stability of Candida antarctica lipase B in ionic liquids. Korean J. Chem. Eng. 25, 291–294 (2008). https://doi.org/10.1007/s11814-008-0051-0
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DOI: https://doi.org/10.1007/s11814-008-0051-0