Abstract
Molasses and starch were used to estimate the effect of co-substrate on anaerobic slurry phase bioremediation of TNT (2,4,6-trinitrotoluene)-contaminated soil. Degradation efficiency of TNT in molasses and starch addition was approximately 97% and 87%, which is 50–60% higher than 38% without co-substrate addition. Molasses and starch addition enhanced TNT degradation. It was proved that the TNT degradation was a mainly biological process from the result of an abiotic control experiment. In case of molasses addition, overall first order degradation rate was 0.0161/day indicating the most active TNT degradation. The first order degradation rates (k1=0.0056 for molasses, k1=0.0033/day for starch) before 60 days were slower than those thereafter (k2=0.0237 for molasses, k2=0.0136/day for starch). Lower degradation rate in early stage might be due to adaptation of native soil microorganisms.
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References
A. Esteve-Nunez and J. L. Ramos, Environ. Sci. Technol., 32, 3802 (1998).
T. Urbanski, Chemistry and technology of explosives, Vol.4. Pergamon Press, Oxford (1983).
G. I. Sunahara, S. Dodard, M. Sarrazin, L. Paquet, J. Hawari, C.W. Greer, G. Ampleman, S. Thiboutot and A.Y. Renoux, Ecotoxicol. Environ. Safety, 43, 138 (1999).
V. Costa, R. Boopathy and J. Manning, Biores. Technol., 56, 273 (1996).
N. R. Adrian and C. M. Arnett, Chemosphere, 66, 1849 (2007).
D. C. Schmelling, K. A. Gray and P.V. Kamat, Wat. Res., 31, 1439 (1997).
S. H. Woo, C.O. Jeon and J. M. Park, Korean J. Chem. Eng., 21, 412 (2004).
USEPA, Bioremediation of hazardous wastes research, development, and field evaluations, EPA/540/R-95/532 (1995).
S. R. Guiot, C. F. Shen, L. Paquet, B. Jerome, J. Hawari, S. Thiboutot and G. Ampleman, Bioremediation of nitroaromatic and haloaromatic compounds, Vol. 7, Alleman, B. C. and Leeson, A., Eds. Battelle Press, Columbus, OH (1999).
N.G. McCormick, J.H. Cornell and A. M. Kaplan, Appl. & Environ. Microbiol., 35, 945 (1978).
D. L. Kaplan and A. M. Kaplan, App. & Environ. Microbiol., 44, 757 (1982).
N.G. McCormick, F. E. Feeherry and H. S. Levinson, App. & Environ. Microbiol., 31, 949 (1976).
P. M. Bradley and F. H. Chapelle, Environ. Sci. & Technol., 29, 802 (1995).
D. Bruns-Nagel, J. Breitung, E. Von Low, K. Steinbach, T. Gorontzy, M. Kahl, K.-H. Blotevogel and D. Gemsa, App. & Environ. Microbiol., 62, 2651 (1996).
P. D. Fiorella and J. C. Spain, Appl. Environ. Microbiol., 63, 2007 (1997).
E. K. Nyer, S. Fam, D. F. Kidd, F. J. Johns II, P. L. Palmer, G. Boettcher, T. L. Crossman and S. S. Suthersan, In situ treatment technology, CRC Press, Boca Raton, NY (1996).
J. L. Osmon and R. E. Klausmeier, Develop. Ind. Microbiol., 14, 247 (1972).
J. B. Eweis, S. J. Ergas, D. P.Y. Chang and Schroeder, Bioremediation principles, McGraw Hill Inc. (1998).
C.W. Park, T. H. Kim, S.Y. Kim, S.W. Kim, J.W. Lee and S. H. Kim, J. Biosci. Bioeng., 95, 567 (2003).
USEPA, Method 8330A: Nitroaromatics and nitroamines by high performance liquid chromatography (HPLC), Test Methods for Evaluating Solid Waste, Physical/Chemical Methods, SW846 Revision 1, Office of Solid Waste, Washington, DC (1998).
USEPA, J.R.Simplot ex-situ anaerobic bioremediation technology: TNT, SITE Technology Capsule, EPA540/R/95/529a (1995).
R. E. Hungate, Methods in microbiology, Norris, J. R. and Ribbons, R.W, Ed. Academic Press, NY (1969).
M. E. Fuller and J. F. Manning, Biores. Technol., 91, 123 (2004).
B. K. Robertson and P. K. Jjemba, Chemosphere, 58, 263 (2005).
R. Boopathy, Soil & Sed. Contami., 25, 495 (2001).
R. Boopathy, J. Hazard. Mater., 92, 103 (2002).
S. D. Comfort, P. J. Shea, L. S. Hundal, Z. Li, B. L. Woodbury, J. L. Martin and W. L. Powers, J. Environ. Qual., 24, 1174 (1995).
P.G. Thorne and D. C. Leggett, Investigations of explosives and their conjugated transformation products in biotreatment matrices, CRREL Special Report 99-3 (1999).
C. Dubois, G. Ampleman, S. Thiboutot, C.W. Greer, J. Hawari and G. Sunahara, Bioremediation of RDX-contaminated soil: A preliminary field trial, Air & Waste Manage. Associ. 90th Annu. Meeting & Exhibi., 97-WP101.07 (1997).
R. C. Loehr, Land disposal of hazardous wastes, hazardous waste management engineering, E. J. Martin and J. H. Johnson, Eds. Van Nostrand Reinhold, NY (1987).
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In, BH., Park, JS., Namkoong, W. et al. Effect of co-substrate on anaerobic slurry phase bioremediation of TNT-contaminated soil. Korean J. Chem. Eng. 25, 102–107 (2008). https://doi.org/10.1007/s11814-008-0018-1
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DOI: https://doi.org/10.1007/s11814-008-0018-1