Abstract
λ-Carrageenan is a highly sulfated polysaccharide alternating of 1,4-O-α-D-galactopyranose-2,6-sulfate (D2S,6S) and 1,3-O-β-D-galactopyranose-2-sulfate (G2S). λ-Carrageenases are desirable tools for λ-carrageenan degradation. Based on the genome mining, a novel λ-carrageenase Cgl150A_Wa was cloned from the bacterium Wenyingzhuangia aestuarii and expressed in Escherichia coli. Cgl150A_Wa was an endo-acting enzyme and exhibited its maximum activity at 30°C and pH 8.0. By employing a glycomics strategy that combined ultra-performance liquid chromatography-mass spectrometry analysis and glycoinformatics, Cgl150A_Wa was proven to degrade λ-carrageenan octaose and hexaose, and the major hydrolysis product of Cgl150A_Wa was λ-carrageenan tetrose. In addition to the typical λ-carrageenan motifs, the active center of Cgl150A_Wa might tolerate desulfated λ-carrageenan motifs. Cgl150A_Wa is a potential biotechnological tool for preparing λ-carrageenan oligosaccharides and structural investigation.
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Acknowledgements
This work was supported by the Fundamental Research Funds for the Central Universities (No. 202012020), and the National Key R&D Program of China (No. 2018YFC 0311203).
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Sun, Y., Cao, S., Zhang, Y. et al. Expression and Characterization of a Novel λ-Carrageenase Cgl150A_Wa from Wenyingzhuangia aestuarii. J. Ocean Univ. China 23, 209–215 (2024). https://doi.org/10.1007/s11802-024-5658-1
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DOI: https://doi.org/10.1007/s11802-024-5658-1