Abstract
Cells from Echinacea purpurea (L.) Moench. (Asteraceae), Exacum affine Balf. f. (Gentianaceae), Melittis melissophyllum L. (Lamiaceae), Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. (Rutaceae) agitating cultures perform a biotransformation reaction on exogenously supplied hydroquinone into its β-D-glucoside — arbutin, product with valuable medicinal and cosmetic properties. The maximum content of arbutin (determined by HPLC) in the biomass from investigated cultures is 4.01; 3.44; 1.79; 2.48 and 5.07 g/100 g d.w., respectively. Nothing but Ammi majus L. (Apiaceae) cultures contain trace amounts of the product. Arbutin is accumulated in cells; it is occasionally found in media only in vestigial amounts. In most of the investigated cultures the efficiency of the biotransformation process is about 60 %.
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Abbreviations
- BAP:
-
6-benzylaminopurine; biomass-shoots, differentiated callus, undifferentiated callus or cell aggregates from suspension
- d.w.:
-
dry weight
- GA3 :
-
gibberellic acid
- HPLC:
-
high performance liquid chromatography
- LS:
-
medium of Linsmaier and Skoog, 1965
- MS:
-
medium of Murashige and Skoog, 1962
- NAA:
-
α-naphthaleneacetic acid
- PLC:
-
preparative layer chromatography
- s.m.:
-
sucha masa
- TLC:
-
thin layer chromatography
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Skrzypczak-Pietraszek, E., Szewczyk, A., Piekoszewska, A. et al. Biotransformation of hydroquinone to arbutin in plant in vitro cultures — preliminary results. Acta Physiol Plant 27, 79–87 (2005). https://doi.org/10.1007/s11738-005-0039-x
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DOI: https://doi.org/10.1007/s11738-005-0039-x