Summary
Young leaf segments from plants growing both in vivo and in vitro were cultured on Murashige and Skoog (MS) medium supplemented with auxins [naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D)], cytokinins [kinetin (KN) and N6-benzyladenine (BA)] and coconut liquid endosperm (CW). The explants from mature leaves did not show any growth and turned necrotic, while those obtained from juvenile leaves growing in vitro developed protocorm-like bodies (PLBs) at their cut surfaces within 4–8 wk depending on the growth medium. An optimum of 18 PLBs developed from leaf explants on medium supplemented with 2.0 mg l−1 (8.87 μM) BA. Upon subculture in basal MS medium, the PLBs differentiated into plantlets within 6–8 wk. The resulting plantlets were successfully transferred to vermiculite initially and subsequently to potting mixture; 84% of the plantlets survived after 3 mo. of transplantation.
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Arditti, J.; Ernst, R. Micropropagation of orchids. New York: John Wiley and Sons; 1993:682 pp.
Champagnat, M.; Morel, G. M.; Mounetou, B. La multiplication vegetative des Cattleya A partir de juenes feuilles cultivees aseptiquement in vitro. Ann. Sci. Nat. Bot. Biol. Veg. Ser. 12, 11:97–114; 1970.
Churchill, M.-E.; Arditti, J.; Ball, E. A. Clonal propagation of orchids from leaf tips. Am. Orchid Soc. Bull. 42:109–113; 1971.
Churchill, M.-E.; Ball, E. A.; Arditti, J. Production of orchid, plants from seedling leaf tips. Orchid Dig. 34:271–273; 1970.
Mathews, V. H.; Rao, P. S. In vitro multiplication, of Vanda hybrids through tissue culture techniques. Plant Sci. Lett. 17:383–389; 1980.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Nayak, N. R.; Patnaik, S.; Rath, S. P. Direct shoot regeneration from foliar explants of an epipytic orchid, Acampe praemorsa (Roxb.), Blatter and McCann. Plant Cell Rep. 16:583–586; 1997.
Rao, A. T. Conservation of wild orchids of Kodagu in the Western Ghats. Bangalore: The Technology Development and Agricultural Technologies and Services Pvt. Ltd.; 1998;242 pp.
Seeni, S. Micropropagation of blue Vanda using leaf bases. Proc. Natl. Seminar on Current Research Trend in Indian Orchids with special reference to tissue culture technology. Shillong, India: NEHU; 1988:22 pp.
Seeni, S.; Latha, P. G. Foliar regeneration of the endengered Red Vanda, Renanthera imschootiana. Plant Cell Tiss. Organ Cult. 29:167–172; 1992.
Tanaka, M.; Sakanishi, Y. Clonal propagation of Phalaenopsis by leaf tissue cultures. Am. Orchid. Soc. Bull. 46:733–737; 1977.
Teng, W. L.; Nicloson, L.; Teng, M. C. Micropropagation of Spathoglotis plicata. Plant Cell Rep. 16:831–835; 1997.
Vacin, E.; Went, F. W. Some pH changes in nutrient solutions. Bot. Gaz. 110:605–613; 1949.
Vij, S. P.; Pathak, P. Micropropagation of orchids through leaf segments. J. Orchid Soc. India 4:69–88; 1990.
Vij, S. P.; Sood, A.; Plaha, K. K. Propagation of Rhynchostylis retusa Bl. (Orchidaceae) by direct organonenesis from leaf segment cultures. Bot. Gaz. 145:210–214; 1984.
Vij, S. P.; Sood, A.; Sharma, M. In vitro leaf segment culture of Vanda testacea (Lindl.) Reichb. F. (=V. parviflora Lindl.) (Orchidaceae). Curr. Sci. 55:1100–1101; 1986.
Yam, T. W.; Weatherhead, M. A. Leaf-tip culture of several native orchids of Hong Kong. Lindlevana 6:147–150; 1991.
Zimmer, K.; Peiper, W. Weitere Untersuchungen zur Kultur in vitro von. Aechmea Gartenbauwissenschaft 40:129–132; 1975.
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Murthy, H.N., Pyati, A.N. Micropropagation of Aerides maculosum lindl. (Orchidaceae). In Vitro Cell.Dev.Biol.-Plant 37, 223–226 (2001). https://doi.org/10.1007/s11627-001-0039-5
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DOI: https://doi.org/10.1007/s11627-001-0039-5