Abstract
Aptamer-functionalized silver nanoclusters (Ag NCs) have been attracting a lot of interest as label-free probes which have been successfully applied to both cell imaging and molecular detection. MUC1 aptamer is an ssDNA aptamer that specifically binds to MUC1 mucin which is a large transmembrane glycoprotein whose expression level increases at least 10-fold in primary and metastatic breast cancers. Using C4A4C3-linker-MUC1 as template, the Ag NCs were synthesized through one-pot process. The fluorescence intensity of Ag NCs was found to be closely related to the length and type (poly adenine or thymine) of the linker, the optimum linker being–AAAAA–. Using the C4A4C3-A5-MUC1 as the scaffold, the synthesized Ag NCs emitted fluorescence with high quantum yield (QY) of 66.5%. Based on the specific interaction between the MUC1 aptamer and MUC1 mucin, the C4A4C3-A5-MUC1-stabilized Ag NCs could recognize and differentiate the MCF-7 breast cancer cells from MDA-MB-231 breast cancer and A549 human lung cancer cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Yeh HC, Sharma J, Han JJ, Martinez JS, Werner JH. Nano Lett, 2010, 10: 3106–3110
Yang SW, Vosch T. Anal Chem, 2011, 83: 6935–6939
Sharma J, Yeh HC, Yoo H, Werner JH, Martinez JS. Chem Commun, 2011, 47: 2294–2296
Lee J, Park J, Lee HH, Kim HI, Kim WJ. J Mater Chem B, 2014, 2: 2616–2621
Yin J, He X, Wang K, Qing Z, Wu X, Shi H, Yang X. Nanoscale, 2012, 4: 110–112
Li J, Zhong X, Cheng F, Zhang JR, Jiang LP, Zhu JJ. Anal Chem, 2012, 84: 4140–4146
Yin J, He X, Wang K, Xu F, Shangguan J, He D, Shi H. Anal Chem, 2013, 85: 12011–12019
Ono A, Cao S, Togashi H, Tashiro M, Fujimoto T, Machinami T, Oda S, Miyake Y, Okamoto I, Tanaka Y. Chem Commun, 2008, 39: 4825
Okamoto I, Iwamoto K, Watanabe Y, Miyake Y, Ono A. Angew Chem Int Ed, 2009, 48: 1648–1651
Megger DA, Fonseca Guerra C, Hoffmann J, Brutschy B, Bickelhaupt FM, Müller J. Chem Eur J, 2011, 17: 6533–6544
Ono A, Torigoe H, Tanaka Y, Okamoto I. Chem Soc Rev, 2011, 40: 5855–5866
Yang X, Gan L, Han L, Wang E, Wang J. Angew Chem Int Ed, 2013, 52: 2022–2026
Zhou XH, Kong DM, Shen HX. Anal Chem, 2010, 82: 789–793
Gwinn EG, O'Neill P, Guerrero AJ, Bouwmeester D, Fygenson DK. Adv Mater, 2008, 20: 279–283
Richards CI, Choi S, Hsiang JC, Antoku Y, Vosch T, Bongiorno A, Tzeng YL, Dickson RM. J Am Chem Soc, 2008, 130: 5038–5039
Li T, He N, Wang J, Li S, Deng Y, Wang Z. RSC Adv, 2016, 6: 22839–22844
Xi Z, Huang R, Li Z, He N, Wang T, Su E, Deng Y. ACS Appl Mater Interfaces, 2015, 7: 11215–11223
Zhao X, Huang Y, Yang CJ, Mao B. Sci China Chem, 2015, 58: 1858–1865
Xi Z, Huang R, Deng Y, He N. J Biomed Nanotechnol, 2014, 10: 3043–3062
Guo F, Hu Y, Yu L, Deng X, Meng J, Wang C, Yang XD. J Nanosci Nanotechnol, 2016, 16: 2246–2253
Sun Z, Wang Y, Wei Y, Liu R, Zhu H, Cui Y, Zhao Y, Gao X. Chem Commun, 2011, 47: 11960–11962
Jiang H, Xu G, Sun Y, Zheng W, Zhu X, Wang B, Zhang X, Wang G. Chem Commun, 2015, 51: 11810–11813
Ludwig JA, Weinstein JN. Nat Rev Cancer, 2005, 5: 845–856
Won JY, Choi JW, Min J. Biosens Bioelectron, 2013, 40: 161–166
Jayasena SD. Clinic Chem, 1999, 45: 1628–1650
Huang R, Xi Z, He N. Sci China Chem, 2015, 58: 1122–1130
Kong RM, Chen Z, Ye M, Zhang XB, Tan WH. Sci China Chem, 2011, 54: 1218–1226
Ferreira CSM, Papamichael K, Guilbault G, Schwarzacher T, Gariepy J, Missailidis S. Anal Bioanal Chem, 2008, 390: 1039–1050
Li J, Jia X, Li D, Ren J, Han Y, Xia Y, Wang E. Nanoscale, 2013, 5: 6131–6138
Sengupta B, Springer K, Buckman JG, Story SP, Abe OH, Hasan ZW, Prudowsky ZD, Rudisill SE, Degtyareva NN, Petty JT. J Phys Chem C, 2009, 113: 19518–19524
Acknowledgments
This work was supported by the National Basic Research Program of China (2014CB744501), the National Natural Science Foundation of China (61271056, 61527806), the Natural Science Foundation of Jiangsu Province (BK20141332), the National Youth Science Foundation of China (61301043), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Fundamental Research Funds for the Central Universities.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Li, T., Yang, J., Ali, Z. et al. Synthesis of aptamer-functionalized Ag nanoclusters for MCF-7 breast cancer cells imaging. Sci. China Chem. 60, 370–376 (2017). https://doi.org/10.1007/s11426-016-0159-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11426-016-0159-2