Abstract
During three rice-growing seasons in Uruguay, field experiments were conducted to study the contribution of cyanobacterial inoculation and chemical N fertilization to rice production. Neither grain yield nor fertilizer recovery by the plant were affected by inoculation with native cyanobacterial isolates. A low fertilizer use efficiency (around 20%) was observed when labelled (NH4)2SO4 was applied at sowing. Recovery of applied 15N by the soil–plant system was 50%. Inoculation did not modify 15N uptake by the plant when the fertilizer was three-split applied either. The total N-fertilizer recovery was higher when the fertilizer was split than when applied in a single dose. Plant N-fertilizer uptake was higher when the fertilizer was applied at tillering. Uptake of 15N from cyanobacteria by rice was studied in a greenhouse pots experiment without chemical nitrogen addition. Recovery of 15N from labelled cyanobacteria by rice in greenhouse growth conditions was similar to that of partial recovery of (NH4)2SO4 applied at sowing in the field. Cyanobacterial N mineralization under controlled conditions was fast as cyanobacterial N was detected in plants after 25 days. Moreover 40 days after inoculation non-planted and inoculated soil had more inorganic N than the non-inoculated one.
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References
Balandreau J, Roger P (1996) Some comments about a better use␣of biological nitrogen fixation in rice cultivation. In: Rahnan␣M, Kumar A, van Hove C, Begum ZNT, Heulin T, Hartmann A (eds) Biological nitrogen fixation associated with rice production. Kluwer Academic Publishers, Dorchdrecht, pp 1–12, ISBN 0–7923 4197-X
Cassman KJ, Peng S, Olk DC, Ladha JK, Reichardt W, Dobermann A, Singh U (1998) Opportunities for increased nitrogen-use efficiency from resource management in irrigated rice systems. Field Crop Res 56:7–39
De Datta S, Obcemea W, Chen R, Calabio J, Evangelista R (1987) Effect of water depth on nitrogen use efficiency and nitrogen-15 balance in lowland rice. Agron J 79:210–216
De Datta SK, Buresh RJ (1989) Integrated nitrogen management in irrigated rice. Adv Soil Sci 10:143–169
Fernández-Valiente E, Quesada A, Prosperi C, Trillo M, Carreres R, Gonzáles T (1996) Contribution of biological nitrogen fixation to rice production in Mediterranean rice fields. In: Rahnan M, KumarA, van Hove C, Begum ZNT, Heulin T, Hartmann A (eds) Biological nitrogen fixation associated with rice production. Kluwer Academic Publishers, Dorchdrecht, pp 147–157, ISBN 0-7923 4197-X
Fernández-Valiente E, Ucha A, Quesada A, Leganés F, Carreres R (2000) Contribution of N2 fixing cyanobacteria to rice production: availability of nitrogen from 15N-labelled cyanobacteria and ammonium sulphate to rice. Plant Soil 221:109–114
Ghosh TK, Saha KC (1997) Effects of inoculation of cyanobacteria on nitrogen status and nutrition of rice (Oryza sativa L.) in an Entisol amended with chemical and organic sources of nitrogen. Biol Fertil Soils 24:123–128
IAEA (2001) Use of isotope and radiation methods in soil and water management and crop nutrition. IAEA-TCS-14, Vienna
Irisarri P (2006) Role of cyanobacteria as biofertilizers, potentials and limitations. In: A handbook of microbial biofertilizers. The Harworth Press Inc, USA. Chap. 16, pp 417–430, ISBN 1-56022-269-9
Irisarri P, Gonnet S, Monza J (2001) Cyanobacteria in Uruguayan rice fields: diversity, nitrogen fixing ability and tolerance to herbicides and combined nitrogen. J Biotechnol 91:95–103
Kundu DK, Ladha JK (1995) Efficient management of soil and biologically fixed N2 in intensively cultivated rice fields. Soil Biol Biochem 27:431–439
Mandal B, Vlek PLG, Mandal LN (1999) Beneficial effects of blue-green algae and Azolla, excluding supplying nitrogen, on wetland ricefields: a review. Biol Fertil Soils 28:329–342
Norman RJ, Guindo D, Wells BR, Wilson CE (1992) Seasonal accumulation and partitioning of nitrogen-15 in rice. Soil Sci Soc Am J 56:1521–1527
Reddy PM, Roger PA (1988) Dynamics of algal populations and acetylene-reducing activity in five rice soils inoculated with blue-green algae. Biol Fertil Soils 6:14–21
Reynaud PA, Metting B (1988) Colonization potential of cyanobacteria on temperate irrigated soils in Washington State, USA. Biol Agric Hortic 5:197–208
Roger PA, Zimmerman WJ, Lumpkin T (1993) Microbiological management of wetland rice fields. In: Baline Meeting F Jr, Dekker M (eds) Soil microbial technologies. Basel Hong Kong, New York, pp 417–455
Roger PA (1996) Fauna. In: Smith WH (ed) Biology and management of the floodwater ecosystem in ricefields. IRRI & ORSTOM Pub., Manila, 57–78, ISBN 971220068X
Roger PA, Ladha JK (1992) Biological N2 fixation in wetland rice fields: estimation and contribution to nitrogen balance. Plant Soil 141:41–55
Sheehy J, Mnzava M, Cassman K, Mitchell P, Pablico P, Robles R, Samonte H (2004) Temporal origen of nitrogen in the grain of irrigated rice in the dry season: the outcome of uptake, cycling, senescence and competition studied using 15N-point placement technique. Field Crop Res 89:337–348
Sinha RP, Häder D-P (1996) Photobiology and ecophysiology of rice field cyanobacteria. Photochem Photobiol 64:887–896
Stevens G, Hefner S, Gladbach T (2001) Effect of nitrogen timming and rates on tillering and yield of non-till water-seeded rice. Commun Soil Sci Plant Anal 32:421–428
Tarlera S, Gonnet S, Irisarri P, Menes J, Fernández A, Paolino G, Travers D, Deambrosi E (2006) Nitrification and denitrification associated with N2O production in a temperate N-fertilized irrigated Uruguayan ricefield. In: Modern multidisciplinary applied microbiology. Exploiting microbes and their interactions. Ed. Wiley-VCH Verlag, pp 416–420, ISBN 3-527-31611-6
Tomaselli L, Giovannetti L (1993) Survival of diazotrophic cyanobacteria in soil. World J Microbiol Biotechnol 9:113–116
Watanabe I (1986) Nitrogen fixation by non-legumes in tropical agriculture with special reference to wetland rice. Plant Soil 90:343–357
Wilson CE, Norman RJ, Wells BR (1989) Seasonal uptake patterns of fertilizer nitrogen applied in split applications to rice. Soil Soc Am J 53:1884–1887
Zaccaro MC (2000) Plant growth-promoting cyanobacteria. In: Proceedings of the 5th International PGPR Workshop, Córdoba, Argentina. http://www.ag.auburn.edu/argentina/pdfmanuscripts/zaccaro.pdf (viewed 8-6-2006)
Acknowledgements
This work was supported by IFS (Grant C-2747) and INIA-FPTA No. 108. We thank V. Berriel for total N analysis and A. Bereta for statistical analysis.
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Irisarri, P., Gonnet, S., Deambrosi, E. et al. Cyanobacterial inoculation and nitrogen fertilization in rice. World J Microbiol Biotechnol 23, 237–242 (2007). https://doi.org/10.1007/s11274-006-9219-0
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DOI: https://doi.org/10.1007/s11274-006-9219-0