Abstract
The interest of fruit breeders in haploids and doubled haploids (DH), lies in the possibility of shortening the time needed to produce homozygous lines compared to conventional breeding. Haplo-diploidization through gametic embryogenesis allows single-step development of complete homozygous lines from heterozygous parents. In a conventional breeding programme, a pure line is developed after several generations of selfing. With fruit crops, characterized by a long reproductive cycle, a high degree of heterozygosity, large size, and, sometimes, self-incompatibility, there is no way to obtain haploidization through conventional methods. This paper reviews the current status of research on doubled haploid production in the main fruit crops: Citrus, Malus domestica, Pyrus communis, Pyrus pyrifolia, Prunus persica, Prunus avium, Prunus domestica, Prunus armeniaca, Vitis vinifera, Actinidia deliciosa, Olea europaea, Morus alba, Actinidia deliziosa, [Musa balbisiana (BB)], Carica papaya, Annona squamosa, Feijoa sellowiana, Opuntia ficus-indica, Eriobotrya japonica.
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Acknowledgements
The author wishes to thank Prof R. Testolin for critically reading the manuscript.
Although only a few research studies on fruit crops in vitro haploidization exists compared to those on herbaceous plants, I nevertheless had to omit some of them, because of the space limitations. My apologies to those authors who have not been mentioned.
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Germanà, M.A. Doubled haploid production in fruit crops. Plant Cell Tiss Organ Cult 86, 131–146 (2006). https://doi.org/10.1007/s11240-006-9088-0
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DOI: https://doi.org/10.1007/s11240-006-9088-0