Abstract
A capsular polysaccharide was isolated from cells of opportunistic bacterial pathogen Acinetobacter baumannii strain MAR 55–66. According to carbohydrate analysis and 1H and 13C NMR spectroscopy, it is composed of heptasaccharide repeating units, which include five l-rhamnose (Rha) residues and one residue each of d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc). For structural analysis of this polysaccharide, the Smith degradation, depolymerization with a recombinant endo-glycosidase from a specific bacteriophage, and selective solvolysis with CF3CO2H were applied. The two last methods resulted in formation of the same linear heptasaccharide, which was subjected to the following chemical modifications: borohydride reduction, β-elimination with alkali, and partial acid hydrolysis. The resulting oligosaccharides were isolated by gel-permeation chromatography, and their structures were established by 1H and 13C NMR spectroscopy including 2D 1H-1H and 1H-13C NMR correlation experiments, as well as high-resolution electrospray ionization mass spectrometry. Based on the data obtained, the structure of the branched heptasaccharide repeating unit containing three Rha residues and one GlcNAc residue in the main chain along with two Rha residues and one GlcA residue in the side chain was established. The polysaccharide studied belongs to a group of structurally similar capsular polysaccharides of A. baumannii, which are built up of branched oligosaccharide repeating units that include four or five l-Rha residues and one residue each of d-GlcA and d-GlcNAc.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
J. J. Kenyon, N. P. Arbatsky, E. L. Sweeney, A. S. Shashkov, M. M. Shneider, A. V. Popova, R. M. Hall, Y. A. Knirel, Int. J. Biol. Macromol., 2019, 128, 101.
J. J. Kenyon, N. P. Arbatsky, E. L. Sweeney, Y. Zhang, S. N. Senchenkova, A. V. Popova, M. M. Shneider, A. S. Shashkov, B. Liu, R. M. Hall, Y. A. Knirel, Int. J. Biol. Macromol., 2020, DOI: https://doi.org/10.1016/j.ijbiomac.2020.11.005.
O. Westphal, K. Jann, Methods Carbohydr. Chem., 1965, 5, 83.
Y. A. Knirel, M. M. Shneider, A. V. Popova, A. A. Kasimova, S. N. Senchenkova, A. S. Shashkov, A. O. Chizhov, Biochemistry (Moscow), 2020, 85, 567.
J. O. Duus, C. H. Gotfredsen, K. Bock, Chem. Rev., 2000, 100, 4589.
V. L. L’vov, A. V. Filatov, A. V. Perepelov, A. M. Shpirt, A. S. Shashkov, A. O. Chizhov, Y. A. Knirel, Mendeleev Commun., 2016, 26, 279.
I. J. Goldstein, G. W. Hay, B. A. Lewis, F. Smith, Methods Carbohydr. Chem., 1965, 5, 361.
P. A. Belyakov, V. I. Kadentsev, A. O. Chizhov, N. G. Kolotyrkina, A. S. Shashkov, V. P. Ananikov, Mendeleev Commun., 2010, 20, 125.
Author information
Authors and Affiliations
Corresponding author
Additional information
The authors are grateful to M. V. Edelshtein (Institute of Antimicrobe Therapy of the Smolensk State Medical University, Russian Ministry of Health, Smolensk) for supplying a sample of A. baumannii strain MAR 55–66.
This work was financially supported by the Russian Science Foundation (Project No. 19-14-00273).
This paper does not contain descriptions of studies on animals or humans.
The authors declare no competing interests.
Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 3, pp. 592–599, March, 2021.
Rights and permissions
About this article
Cite this article
Arbatsky, N.P., Shashkov, A.S., Chizhov, A.O. et al. Structure of the capsular polysaccharide of Acinetobacter baumannii MAR 55–66. Russ Chem Bull 70, 592–599 (2021). https://doi.org/10.1007/s11172-021-3130-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11172-021-3130-6