Abstract
IsiA is a membrane-bound Chl a-antenna protein synthesized in cyanobacteria under iron deficiency. Since iron deficiency is a common nutrient stress in significant fractions of cyanobacterial habitats, IsiA is likely to be essential for some cyanobacteria. However, the role it plays in cyanobacteria is not fully understood. In this review paper, we summarize the research efforts directed towards characterizing IsiA over the past three decades and attempt to bring all the pieces of the puzzle together to get a more comprehensive understanding of the function of this protein. Moreover, we analyzed the genomes of over 390 cyanobacterial strains available in the JGI/IMG database to assess the distribution of IsiA across the cyanobacterial kingdom. Our study revealed that only 125 such strains have an IsiA homolog, suggesting that the presence of this protein is a niche specific requirement, and cyanobacterial strains that lack IsiA might have developed other mechanisms to survive iron deficiency.
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Abbreviations
- CBP:
-
chlorophyll-binding proteins
- Chl a :
-
chlorophyll a
- EET:
-
excitation energy transfer
- FMO:
-
Fenna-Mathews-Olson protein
- Fur:
-
ferric uptake regulator
- Fv/Fm :
-
maximal photochemical efficiency of PSII
- GFP:
-
green fluorescent protein
- Hilp:
-
high light-inducible proteins
- HNLC:
-
high-nitrate low-chlorophyll
- IR:
-
inverted repeat
- IsiA:
-
iron-stress-induced protein A
- IsiB:
-
iron-stress-induced protein B
- IdiA:
-
iron-deficiency-induced protein A
- IdiB:
-
iron-deficiency-induced protein B
- IsrR:
-
iron stress-repressed RNA
- NPQ:
-
nonphotochemical quenching
- PBS:
-
phycobilisome
- Pcb:
-
prochlorophyte chlorophyll a/b protein
- PerR:
-
peroxide operon regulator
- ROS:
-
reactive oxygen species
- σPSI :
-
effective cross-section of the photosynthetic reaction center PSI
- UCYN-A:
-
noncultivated unicellular cyanobacteria of group A
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Acknowledgements: We thank members of the Pakrasi research group for collegial discussions. Supported by funding from Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC 0001035; the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy, under grant number DE-FG02- 99ER20350; and Gordon and Betty Moore Foundation through Grant GBMF5760 to HBP.
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Chen, HY., Bandyopadhyay, A. & Pakrasi, H.B. Function, regulation and distribution of IsiA, a membrane-bound chlorophyll a-antenna protein in cyanobacteria. Photosynthetica 56, 322–333 (2018). https://doi.org/10.1007/s11099-018-0787-7
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DOI: https://doi.org/10.1007/s11099-018-0787-7