The distribution of nitroxidergic neurons in the medulla oblongata nuclei in Wistar rats (n = 8) was studied using histochemical (NADPH-diaphorase) and immunohistochemical methods with antisera to the neuronal form of nitric oxide synthase (nNOS). NADPH-diaphorase was found in large and small neurons in the sensory, autonomic, and motor nuclei. The latter were found to contain particularly large numbers of neurons expression this enzyme activity. In contrast to NADPH-diaphorase, nNOS in the corresponding nuclei was always present in smaller quantities of mainly smaller neurons. The sensory nuclei (solitary tract nucleus, reticular parvocellular and lateral nuclei, spinal nucleus of the trigeminal nerve) showed 1.5–3 times more nNOS neurons than the motor nuclei. In some nuclei (the nucleus ambiguus, the nucleus of the hypoglossal nerve) containing large numbers of NADPH-diaphorase-positive neurons, immunoreactive cells were particularly rare.
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Translated from Morfologiya, Vol. 144, No. 6, pp. 9–14, November–December, 2013.
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Chertok, V.M., Kotsyuba, A.E. Distribution of NADPH-Diaphorase and Neuronal NO Synthase in the Nuclei of the Medulla Oblongata in Rats. Neurosci Behav Physi 44, 909–913 (2014). https://doi.org/10.1007/s11055-014-0001-8
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DOI: https://doi.org/10.1007/s11055-014-0001-8