Abstract
Methane oxidation in the cover soil of the Khmet'evo municipal landfill in Moscow oblast was investigated. Methane emission from the experimental site of the landfill was highly heterogeneous. At a depth of 45–60 cm, the pore gas mainly consisted of CH4 (60–70%) and CO2 (30–40%). In the upper layers of the cover soil, the concentration of these gases sharply decreased. Methods for estimation of the methane-oxidizing activity in the cover soil of the landfill were tested. The rate of methane oxidation in the soil correlated with the cell number of culturable methanotrophic bacteria and was the factor limiting methane emission from the surface of the landfill. The method of indirect immunofluorescence revealed ten known species of methanotrophic bacteria in enrichment cultures obtained from samples of the cover soil. Our results also indicate the presence of unknown psychrotolerant methanotrophs that are active at the low temperatures characteristic of Moscow oblast.
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REFERENCES
IPCC, Climate Change 2001: The Scientific Basis. Contribution of Working Group I to the Third Assessment Report of the Intergovernmental Panel on Climate Change, Houghton, J.T. et al., Eds., Cambridge: Cambridge Univ. Press, 2001, p. 881.
Nozhevnikova, A.N. and Lebedev, V.S., Burial Sites of Municipal Garbage as a Source of Atmospheric Methane, J. Ecol. Chem., 1995, vol. 4, no.1, pp. 48–58.
Bogner, J., Meadows, M., and Czepiel, P., Fluxes of Methane between Landfills and the Atmosphere: Natural and Engineered Controls, Soil Use Manage, 1997, vol. 13, pp. 268–277.
Lebedev, V.S., Gorbatyuk, O.V., Ivanov, D.V., Nozhevnikova, A.N., and Nekrasova, V.K., Biogeochemical Processes of the Production and Oxidation of Biogas at Burial Sites of Municipal Garbage, Zh. Ekol. Khim., 1993, no. 4, pp. 323–334.
Nozhevnikova, A., Kallistova, A., Kevbrina, M., Glagolev, M., Nekrasova, V., Lebedev, V., Einola, J., Sormunen, K., and Rintala, J., Oxidation of Methane in Landfills Located in Cold Climate Zone: Geochemical and Microbiological Studies, Proc. Int. Workshop on Sustainable Landfill Management, Chennai, India, Dec. 2003, pp. 183–190.
Orlov, D.S., Min'ko, O.I., Kasparov, S.V., Amosova, Ya.M., and Glagolev, M.V., Methods for Studying the Gas Function of Soils, Sovremennye fizicheskie i khimicheskie metody issledovaniya pochv (Modern Physical and Chemical Methods for Investigation of Soils), Voronin, A.D. and Orlov, D.S., Eds., Moscow: Mosk. Gos. Univ., 1987.
Gal'chenko, V.F., Metanotrofnye bakterii (Methanotrophic Bacteria), Moscow: GEOS, 2001.
Alexander, M., Most Probable Number Method for Microbial Populations, Methods of Soil Analysis, Page, A.L., Ed., Madison, Wisconsin, 1982, pp. 815–820.
Gal'chenko, V.F., Abramochkina, F.N., Bezrukova, L.V., Sokolova, E.N., and Ivanov, M.V., The Species Composition of Aerobic Methanotrophic Microflora of the Black Sea, Mikrobiologiya, 1988, vol. 57, no.2, pp. 305–311.
Nozhevnikova, A., Glagolev, M., Nekrasova, V., Einola, J., Sormunen, K., and Rintala, J., The Analysis of Methods for Measurement of Methane Oxidation in Landfills, Water Sci. Technol., 2003, vol. 48, no.4, pp. 45–52.
Kallistova, A.Yu., Glagolev, M.V., Shnyrev, N.A., Kevbrina, M.V., Nekrasova, V.K., Chistotin, M.V., Faustova, E.V., Serebryanaya, M.I., and Nozhevnikova, A.N., Methane Emission from the Surface of a Municipal Landfill as Dependent on the Age of the Landfill and the Season, Zh. Ekol. Khim. (in press).
Hilger, H. and Humer, M., Biotic Landfill Cover Treatments for Mitigating Methane Emissions, Environ. Monitor. Assess., 2003, vol. 84, pp. 71–84.
Nozhevnikova, A.N., Nekrasova, V.K., Lebedev, V.S., and Lifshits, A.B., Microbiological Processes in Landfills, Water Sci. Technol., 1993, vol. 27, no.2, pp. 243–252.
Boeckx, P. and Van Cleemput, O., Methane Oxidation in Neutral Landfill Cover Soil: Influence of Moisture Content, Temperature, and Nitrogen-Turnover, J. Environ. Qual., 1996, vol. 25, pp. 178–183.
Bogner, J.E., Spokas, K.A., and Burton, E.A., Temporal Variations in Greenhouse Gas Emissions at Midlatitude Landfill, J. Environ. Qual., 1999, vol. 28, pp. 278–288.
Jones, H.A. and Nedwell, D.B., Methane Emission and Methane Oxidation in Landfill Cover Soil, FEMS Microbiol. Ecol., 1993, vol. 102, pp. 185–195.
Nozhevnikova, A.N., Nekrasova, V.K., and Lebedev, V.S., Low-Temperature Production and Oxidation of Methane by the Microflora of Sludge Checks, Mikrobiologiya, 1999, vol. 68, no.2, pp. 267–272.
Nozhevnikova, A.N., Nekrasova, V.K., Kevbrina, M.V., and Kotsyurbenko, O.R., Production and Oxidation of Methane at Low Temperature by the Microbial Population of Municipal Sludge Checks Situated in North-East Europe, Water Sci. Technol., 2001, vol. 44, no.4, pp. 89–95.
Dedysh, S.N., Khmelenina, V.N., Suzina, N.E., Trotsenko, Yu.A., Semrau, J.D., Liesack, W., and Tiedje, J.M., Methylocapsa acidiphila gen. nov., sp. nov., a Novel Methane-Oxidizing and Dinitrogen-Fixing Acidophilic Bacterium from Sphagnum Bog, Int. J. Syst. Evol. Microbiol., 2002, vol. 52, pp. 251–261.
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Translated from Mikrobiologiya, Vol. 74, No. 5, 2005, pp. 699–706.
Original Russian Text Copyright © 2005 by Kallistova, Kevbrina, Nekrasova, Glagolev, Serebryanaya, Nozhevnikova.
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Kallistova, A.Y., Kevbrina, M.V., Nekrasova, V.K. et al. Methane Oxidation in Landfill Cover Soil. Microbiology 74, 608–614 (2005). https://doi.org/10.1007/s11021-005-0110-z
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DOI: https://doi.org/10.1007/s11021-005-0110-z