Abstract
Fifty-three strains of Saturn-spored yeasts were analyzed by means of restriction analysis of the amplified fragment of rDNA comprising the 5.8S rRNA gene and the internal transcribed spacers ITS1 and ITS2. The use of endonucleases HaeIII and MspI enabled clear differentiation of yeast species Williopsis mucosa, W. salicorniae, Zygowilliopsis californica, and Komagataea pratensis and the Williopsis sensu stricto complex. The minisatellite primer M13 was proposed for differentiation between sibling species of Williopsis sensu stricto, which have identical restriction profiles. PCR with primer M13 enabled reidentification of a number of collection strains, species identification of Saturn-spored isolates from the Far East, and detection of three strains affiliated to novel taxa. The latter have unique PCR profiles and differ in the nucleotide sequences of ITS1 and ITS2 fragments of rDNA. Possible variations in the results obtained with different molecular methods are discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
REFERENCES
P. Valente J.P. Ramos O. Leoncini (1999) ArticleTitleSequencing As a Tool in Yeast Molecular Taxonomy Can. J. Microbiol. 45 949–958
C.P. Kurtzman C.J. Robnett (1998) ArticleTitleIdentification and Phylogeny of Ascomycetous Yeasts from Analysis of Nuclear Large Subunit (26S) Ribosomal DNA Partial Sequences Antonie van Leeuwenhoek 73 331–371 Occurrence Handle10.1023/A:1001761008817 Occurrence Handle1:CAS:528:DyaK1MXjtFOjsg%3D%3D Occurrence Handle9850420
D. Dlauchy J. Tornai-Lehoczki G. Peter (1999) ArticleTitleRestriction Enzyme Analysis of PCR Amplified rDNA as a Taxonomic Tool in Yeast Identification Syst. Appl. Microbiol. 22 445–453
B. Esteve-Zarzoso C. Belloch F. Uruburu A. Querol (1999) ArticleTitleIdentification of Yeasts by RFLP Analysis of the 5.8S rRNA Gene And the Two Ribosomal Internal Transcribed Spacers Int. J. Syst. Bacteriol. 49 329–337
Kurtzman, C.P., Williopsis Zender, in The Yeasts. A Taxonomic Study, Kurtzman, C.P. and Fell, J.W., Eds., Elsevier, 1998, pp. 413–419.
Z. Liu C.P. Kurtzman (1991) ArticleTitlePhylogenetic Relationships among Species of Williopsis and Saturnospora gen. nov. as Determined from Partial rRNA Sequences Antonie van Leeuwenhoek 60 21–30
Y. Yamada M. Matsuda K. Maeda C. Sakakibara K. Mikata (1994) ArticleTitleThe Phylogenetic Relationships of the Saturn-Shaped Ascospore-Forming Species of Genus Williopsis Zender and Related Genera Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae): the Proposal of Komagataea gen. nov. Biosci. Biotechnol. Biochem. 58 IssueID7 1236–1244
Y. Yamada J.-I. Yano M. Matsuda T. Higashi K. Mikata (1995) ArticleTitleThe Phylogeny of Williopsis salicorniae Hinzelin, Kurtzman et Smith Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae) Biosci. Biotechnol. Biochem. 5 945–948
S.A. James I.N. Roberts M.D. Collins (1998) ArticleTitlePhylogenetic Heterogeneity of the Genus Williopsis as Revealed by 18S rRNA Gene Sequences Int. J. Syst. Bacteriol. 48 591–596
G.I. Naumov M.M. Vustin I.P. Bab’eva (1980) ArticleTitleSexual Divergence of Yeasts of the Genera Williopsis Zender, Zygowilliopsis Kudriavzev and Hansenula H. et R. Sydow Dokl. Akad. Nauk SSSR 255 IssueID2 468–471
Naumov, G.I., Genosystematics of Yeasts of the Genera Williopsis Zender and Zygowilliopsis Kudriavzev, Mol. Genet. Mikrobiol. Virusol., 1987, no. 2, pp. 3–7.
Vustin, M.M. and Naumov, G.I., Anomalous Meiosis in Hybrids of Biological Species of the Genus Williopsis, Biol. Nauki, 1974, no. 7, pp. 88–91.
Vustin, M.M., Naumov, G.I., Bab’eva, I.P., and Naumova, T.I., Genosystematics of the Yeasts Williopsis saturnus: New Biological Species, Dokl. Akad. Nauk SSSR, 1982, vol. 267, no. 6, pp. 1481–1484.
G.I. Naumov M.M. Vustin T.I. Naumova (1981) ArticleTitleA Genetic Study of the Yeast Species Zygowilliopsis californica, Williopsis saturnus, W.beijerinckii comb. nov., W. mrakii comb. nov. Dokl. Akad. Nauk SSSR 259 IssueID3 718–722
G.I. Naumov M.M. Vustin I.P. Bab’eva I.S. Reshetova (1985) ArticleTitleAddendum to the Genosystematics of the Yeasts of the genus Williopsis and Zygowilliopsis Mikrobiologiya 54 IssueID2 239–244
C.P. Kurtzman (1987) ArticleTitlePrediction of Biological Relatedness among Yeasts from Comparisons of Nuclear DNA Complementarity Studies Mycol. 30 459–468
C.P. Kurtzman (1991) ArticleTitleDNA Relatedness among Saturn-Spored Yeasts Assigned to the Genera Williopsis and Pichia Antonie van Leeuwenhoek 60 13–19
G.I. Naumov N.G. Tokareva E.S. Naumova I.P. Bab’eva (2000) ArticleTitleDiscrimination between the Soil Yeast Species Williopsis saturnus and Williopsis suaveolens by the Polymerase Chain Reaction with the Universal Primer N21 Mikrobiologiya 69 IssueID2 280–285
T.J. White T. Bruns E. Lee J. Taylor (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics PCR Protocols: A Guide to Methods and Applications Academic New York 315–322
E.S. Naumova N.G. Tokareva G.I. Naumov (2001) ArticleTitleWilliopsis saturnus and Williopsis beijerinckii Are Recognized as Distinct Taxa by Means of the Polymerase Chain Reaction with Nonspecific Primers Mikrobiologiya 70 IssueID3 365–369
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Mikrobiologiya, Vol. 73, No. 6, 2004, pp. 768–776.
Original Russian Text Copyright © 2004 by Naumova, Gazdiev, Naumov.
Rights and permissions
About this article
Cite this article
Naumova, E.S., Gazdiev, D.O. & Naumov, G.I. Molecular divergence of the soil yeasts Williopsis sensu stricto. Microbiology 73, 658–665 (2004). https://doi.org/10.1007/s11021-005-0007-x
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11021-005-0007-x