Summary
The important thermochemistry research method-microcalorimetry was successfully applied to study the effect of different nucleotide mutations in -35 box sequence on the gene promoter activity in <Emphasis Type=”Italic”>Escherichia coli</Emphasis>. The metabolic thermogenic curves of different <Emphasis Type=”Italic”>Escherichia coli</Emphasis> strains were obtained by microcalorimetric measurement using the TAM air Isothermal Microcalorimeter. By analyzing the data such as metabolic rate constant (<Emphasis Type=”Italic”>k</Emphasis><Subscript>m</Subscript>), inhibitory ratio (<Emphasis Type=”Italic”>I</Emphasis>) and half-inhibitory concentration of antibiotic (<Emphasis Type=”Italic”>IC</Emphasis><Subscript>50</Subscript>) obtained by the microcalorimetric method, it was found that the structure of -35 box sequence was very important for the gene promoter activity in <Emphasis Type=”Italic”>Escherichia coli</Emphasis>. Different nucleotide mutations in -35 box sequence had different effect on the gene promoter activity.
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Shen, P. Study on the nucleotide mutation in -35 box sequence of gene promoter by means of microcalorimetric method. J Therm Anal Calorim 79, 645–648 (2005). https://doi.org/10.1007/s10973-005-0590-0
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DOI: https://doi.org/10.1007/s10973-005-0590-0