Abstract
Bio-effects of static magnetic fields on cell growth and cell death have been investigated in suspension-cultured tobacco cells as undifferentiated, embryonic plant cell model. The cells in their logarithmic growth phase were exposed to static magnetic field with the magnitudes of 10 mT and 30 mT for 5 h/day. Exposure to static magnetic field ceased the growth and caused an increase in cell death of exposed tobacco cells compared to those cells which were not treated with the field. Promotion of cell death was accompanied by a harmonized increase in the activity of peroxidase and increase of lignifcation of cell walls.
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Amaya, I., Botella, M. A., Calle, M., Medina, M. I., Heredia, A., Bressan, R. A., Hasegawa, P. M., Quesada, M. A., & Valpuesta, V. (1999). Improved germination under osmotic stress or tobacco plants overexpressing a cell wall peroxidase. FEBS Letters, 457, 80–84.
Ames, B. N. (1983). Dietary carcinogens and anti-carcinogens. Oxygen radicals and degenerative diseases. Science, 221, 1256–1264.
Box, H. C., & MacCubbin, A. C. (1997). Lipid peroxidation and DNA damage. Nutrition, 13(10), 920–921.
Dat, J. F., Van Montagu, M., Inze, D., & Van Breusegem, F. (2001). Catalasedeficient tobacco plants: Tools for in planta studies on the role of hydrogen peroxide. Redox Report, 6, 37–42.
Fukuda, H., & Komamine, A. (1982). Lignin synthesis and its related enzymes as markers of tracheary-element differentiation in single cells isolated from the mesophyll of Zinnia elegans. Planta, 155, 423–430.
Ghanati, F., Morit A., & Yokota H. (2001). Selection and partial characterization of a boron-tolerant tobacco cell line. Soil Science and Plant Nutrition, 47, 405–410.
Ghanati, F., Morit A., & Yokota, H. (2005). Effect of aluminum on the growth of tea plant and activation of antioxidant system. Plant Soil, 276, 133–141.
Green, L. M., Miller, A. B., Agnew, D. A., Greenberg, M. L., Li, J., Villeneuve, J. P., & Tibshirani, R. (1999). Childhood leukemia and personal monitoring of residential exposures to electric and magnetic fields in Ontario, Canada. Cancer Causes and Control, 10, 233–243.
Grissom, C. B. (1995). Magnetic field effects in biology: A survey of possible mechanisms with emphasis on radical pair recombination. Chemical Reviews, 95, 3–24.
Hisamitsu, T., Narita, K., Kasahara, T., Seto, A., Yu, Y., & Asano, K. (1997). Induction of apoptosis in human leukemic cells by magnetic fields. The Japanese Journal of Physiology, 47, 307–310.
Iiyama, K., & Wallis, A. (1990). Determination of lignin in herbaceous plants by an improved acetyl bromide procedure. Journal of the Science of Food and Agriculture, 51, 145–161.
Ishisaka, R., Kanno, T., Inai, Y., Nakahara, H., Akiyama, J., Yoshioka, T., & Utsumi, K. (2000). Effects of a magnetic fields on the various functions of subcellular organelles and cells. Pathophysiology, 7, 149–152.
Kroemer, G., Petit, P., Zamzani, N., Vayssiere, J. L., & Mignotte, B. (1995). The biochemistry of programmed cell death. The FASEB Journal, 9, 1277–1287.
Singh, N., & Lai, H. (1998). 60 Hz magnetic field exposure induces DNA crosslinks in rat brain cells. Mutation Research, 400(1–2), 313–320.
Luciana, D., & Luigi, A. (2005). Bioeffects of moderate-intensity static magnetic fields on cell cultures. Micron, 36, 195–217.
Meneghini, R. (1997). Iron homeostasis, oxidative stress, and DNA damage. Free Radical Biology and Medicine, 23, 783–792.
Miyakoshi, J. (2005). Effects of static magnetic fields at the cellular level. Progress in Biophysics and Molecular Biology, 87, 213–223.
Morita, A., Yokota., H., Rahmati Ishka, M., & Ghanati, F. (2006). Changes I peroxidase activity and lignin content of cultured tea cells in response to excess manganese. Soil Science and Plant Nutrition, 52, 26–31.
Normanly, J., Slovin, J. P., & Cohen, J. D. (1995). Rethinking auxin biosynthesis and metabolism. Plant Physiology, 107, 323–329.
Pandolfini, T., Gabbrielli, R., & Comparini, C. (1992). Nickel Toxicity and Peroxidase Activity in Seedlings of Triticum aestivum L Plant, Cell and Environment, 15, 719–275.
Rakosy-Tican, L., Aurori, C. M., & Morariu, V. V. (2005). Influence of near null magnetic field on in␣vitro growth of potato and wild solanum species. Bioelectromagnetics 26, 548–557.
Phillips, J. L., Winters, W. D., & Rutledge, L. (1986). In vitro exposure to electromagnetic fields: Changes in tumour cell properties. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine, 49(3), 463–469.
Renvoitze, C., Biola, A., Pallardy, M., & Breard, J. (1998). Apoptosis: Identification of dying cells. Cell Biology and Toxicology, 14, 111–120.
Robison, J. G., Pendleton, A. R., Monson, K. O., Murray, B. K., & O’Neill, K. L. (2002). Decreased DNA repair rates and protection from heat induced apoptosis mediated by electromagnetic field exposure. Bioelectromagnetics, 23, 106–112.
Sabo, J., Mirossay, L., Horovcak, L., Sarissky, M., Mirossay, A., & Mojzis, J. (2001). Effects of static magnetic field on human leukemic cell line HL-60. Bioelectrochemistry, 56, 227–231.
Schnabelrauch, L. S., Kieliszewski, M., Upham, B. L., Alizedeh, H., & Lamport, D. T. A. (1996). Isolation of pI 4.6 extensin peroxidase from tomato cell suspension cultures and identification of Val-Tyr-Lys as putative intermolecular cross-link site. Plant J, 9, 477–489.
Whetten, R. W., MacKay, J. J., & Sederoff, R. R. (1998). Recent advances in understanding lignin biosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 585–609.
Wyllie, A. H., Kerr, J. F., & Currie, A. R. (1980). Cell death. The significance of apoptosis. International Review of Cytology, 68, 251–306.
Yano, A., Ohashi, Y., Hirasaki, T., & Fujiwara, K. (2004). Effects of a 60 Hz magnetic field on photosynthetic CO2 uptake and early growth of radish seedlings. Bioelectromagnetics, 25, 572–581.
Zhang, Q. M., Tokiwa, M., Doi, T., Nakahara, T., Chang, P. W., Nakamura, N., Hori, M., Miyakoshi, J., & Yonei, S. (2003). Strong static magnetic field and the induction of mutations through elevated production of reactive oxygen species in Escherichia coli soxR. International Journal of Radiation Biology, 79, 281–286.
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Abdolmaleki, P., Ghanati, F., Sahebjamei, H. et al. Peroxidase activity, lignification and promotion of cell death in tobacco cells exposed to static magnetic field. Environmentalist 27, 435–440 (2007). https://doi.org/10.1007/s10669-007-9080-1
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DOI: https://doi.org/10.1007/s10669-007-9080-1