Abstract
Lignin is closely related to the lodging resistance of common buckwheat (Fagopyrum esculentum Moench.). However, the characteristics of lignin synthesis related genes have not yet been reported. We investigated the lignin biosynthesis gene expression, activities of related enzymes, and accumulation of lignin monomers during branching stage, bloom stage, and milky ripe stage by real-time quantitative PCR, UVspectrophotometry, and gas chromatography-mass spectrometry in the 2nd internode of three common buckwheat cultivars with different lodging resistance. The results showed that lignin content and the activity of phenylalanine ammonia lyase (PAL), 4-coumarate: CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD) were closely related to the lodging resistance of common buckwheat. Further, we studied gene expression of cinnamate 4-hydroxylase (C4H), caffeoyl-CoA O-methyltransferase (CCoAOMT), ferulate 5-hydroxylase (F5H), cinnamoyl-CoA reductase (CCR), and caffeic acid O-methyltransferase (COMT). The lignin biosynthesis genes were divided into three classes according to their expression pattern: 1) expression firstly increasing and then descending (PAL, 4CL, CAD, C4H, CCoAOMT, F5H, and CCR), 2) expression remaining constant during maturation (C3H), and 3) expression decreasing with maturation (COMT). The present study provides preliminary insights into the expression of lignin biosynthesis genes in common buckwheat, laying a foundation for further understanding the lignin biosynthesis.
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Abbreviations
- CAD:
-
cinnamyl alcohol dehydrogenase
- CCR:
-
cinnamoyl-CoA reductase
- CCoAOMT:
-
caffeoyl-CoA O-methyltransferase
- C3H:
-
p-coumarate 3-hydroxylase
- C4H:
-
cinnamate 4-hydroxylase
- 4CL:
-
4-coumarate: CoA ligase
- COMT:
-
caffeic acid O-methyltransferase
- F5H:
-
ferulate 5-hydroxylase
- PAL:
-
phenylalanine ammonia lyase
- qPCR:
-
quantitative PCR
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Acknowledgements: This study was supported by the Fundamental Research Funds for the Central Universities (No. XDJK2016D023). The first two authors contributed equally to this paper.
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Hu, D., Liu, X.B., She, H.Z. et al. The lignin synthesis related genes and lodging resistance of Fagopyrum esculentum . Biol Plant 61, 138–146 (2017). https://doi.org/10.1007/s10535-016-0685-4
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DOI: https://doi.org/10.1007/s10535-016-0685-4