Abstract
Ascorbic acid (AsA) is naturally occurring compound with antioxidant activity and plays a pivotal role in plant cell adaptation to salinity stress. The objective of this work was to assess the influence of exogenous AsA on the embryogenic callus of indica rice (Oryza sativa L.) cv. MRQ74 cultivated under saline conditions. NaCl (200 mM) decreased callus fresh and dry masses, relative growth rate, and K+ and Ca+2 content, and increased Na+ content and Na+/K+ ratio. Application of AsA (0.5 or 1 mM) alleviated these effects of salinity. Activities of peroxidase, catalase, superoxide dismutase, as well as content of proline increased due to the NaCl treatment, and these parameters were mostly further increased by 0.5 mM AsA. Thus, AsA can increase callus tolerance to NaCl stress.
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Abbreviations
- ABA:
-
abscisic acid
- APX:
-
ascorbate peroxidase
- AsA:
-
ascorbic acid
- BAP:
-
6-benzylaminopurine
- CAT:
-
catalase
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- ET:
-
ethylene
- GA3 :
-
gibberellic acid
- JA:
-
jasmonic acid
- MS:
-
Murashige and Skoog
- POD:
-
peroxidase
- PVP:
-
polyvinylpyrrolidone
- RGR:
-
relative growth rate
- ROS:
-
reactive oxygen species
- SA:
-
salicylic acid
- SOD:
-
superoxide dismutase
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Acknowledgements: This study is a part of the Ph.D. programme of the first author. This project was financed by Al-Muthanna University, the Ministry of Higher Education and Scientific Research, Iraq, the University Kebangsaan, Malaysia, and the Ministry of Higher Education, Malaysia.
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Alhasnawi, A.N., Che Radziah, C.M.Z., Kadhimi, A.A. et al. Enhancement of antioxidant enzyme activities in rice callus by ascorbic acid under salinity stress. Biol Plant 60, 783–787 (2016). https://doi.org/10.1007/s10535-016-0603-9
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DOI: https://doi.org/10.1007/s10535-016-0603-9