Abstract
Plant vacuoles play several roles in controlling development, pathogen defence, and stress response. γVPE is a vacuolarlocalised cysteine protease with a caspase-1 like activity involved in the activation and maturation of downstream vacuolar hydrolytic enzymes that trigger hypersensitive cell death and tissue senescence. This work provides evidence that γVPE is strongly expressed in Arabidopsis guard cells and is involved in water stress response. The γvpe knock-out mutants showed reduced stomatal opening and an increased resistance to desiccation suggesting a new role of γVPE in control of stomatal movements.
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Abbreviations
- ABA:
-
abscisic acid
- RT-qPCR:
-
real-time quantitative polymerase chain reaction
- RWC:
-
relative water content
- VPE:
-
vacuolar processing enzyme
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Acknowledgments: This work was supported by grants from the “Fondo per la Promozione di Accordi Istituzionali”, project BIOGESTECA 15083/RCC. A. Albertini and F. Simeoni contributed equally to this work.
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Albertini, A., Simeoni, F., Galbiati, M. et al. Involvement of the vacuolar processing enzyme γVPE in response of Arabidopsis thaliana to water stress. Biol Plant 58, 531–538 (2014). https://doi.org/10.1007/s10535-014-0417-6
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DOI: https://doi.org/10.1007/s10535-014-0417-6