Abstract
A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm−3 zeatin, 0.1 mg dm−3 indoleacetic acid and 10 g dm−3 sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0–2.0 mg dm−3 gibberellic acid and 4.0–8.0 mg dm−3 AgNO3, which promoted shoots elongation. The genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers. The results indicated that almost no somaclonal variation was detected among the regenerants.
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Abbreviations
- GA3 :
-
gibberellic acid
- IAA:
-
indoleacetic acid
- MS medium:
-
Murashige and Skoog medium
- RAPD:
-
random amplified polymorphic DNA
- SSR:
-
simple sequence repeat
- ZT:
-
zeatin
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Acknowledgements
This work was partly supported by the grants to Y.-D. Guo (2009CB119000, 2007BAD57B04 and Chinese Universities Scientific Fund 2009-2-06). The first two authors contributed equally to this work.
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Xing, Y., Yu, Y., Luo, X. et al. High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena . Biol Plant 54, 231–236 (2010). https://doi.org/10.1007/s10535-010-0041-z
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DOI: https://doi.org/10.1007/s10535-010-0041-z