Abstract
A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production ~10-fold at ~60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.
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Kwon, RJ., Kim, S.K., Lee, SI. et al. Artificial Transcription Factors Increase Production of Recombinant Antibodies in Chinese Hamster Ovary Cells. Biotechnol Lett 28, 9–15 (2006). https://doi.org/10.1007/s10529-005-4680-7
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DOI: https://doi.org/10.1007/s10529-005-4680-7