Abstract
Adventitious roots from leafstalks and lateral roots were obtained directly from explants of Panax notoginseng. The lateral root explants were more sensitive to the induction of adventitious roots using indole-3-butyric acid. HPLC analysis of saponins extracted from the adventitious roots indicated that several protopanaxatriol saponins were present but ginsenoside Rd was missing, compared with the saponins extracted from the raw herbs. The dry weight of primary adventitious root culture of Panax notoginseng increased 5.25 times during multiplication in a classical shaking-flask system, suggesting that it is a culture system with great potential for scale-up.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
I Asaka I Li M Hirotani Y Asada T Furuya (1993) ArticleTitleProduction of ginsenoside saponins by culturing ginseng (Panax ginseng) embryogenic tissues in bioreactors Biotechnol. Lett. 15 1259–1264 Occurrence Handle1:CAS:528:DyaK2cXhs1WlurY%3D
EJ Hahn YS Kim KW Yu CS Jeong KY Paek (2003) ArticleTitleAdventitious root cultures of Panax ginseng C.V. Meyer and ginsenoside production through large-scale bioreactor system J. Plant Biotechnol. 5 1–6
J Han JJ Zhong (2002) ArticleTitleHigh density cell culture of Panax notoginseng for production of ginseng saponin and polysaccharide in an airlift bioreactor Biotechnol. Lett. 24 1927–1930 Occurrence Handle1:CAS:528:DC%2BD38Xotleku7c%3D
W Jia WY Gao (2003) ArticleTitleTherapeutic transdermal agents for pain containing Chinese medicinal herbs J. Plant Biotechnol. 5 7–11
GJD Klerk B Arnholdt-Schmitt R Lieberei KH Neumann (1997) ArticleTitleRegeneration of roots, shoots and embryos: physiological, biochemical and molecular aspects Biol. Plantarum 39 53–66
G Payane V Bringi C Prince ML Shuler (1991) The quest for commercial production of chemicals from plant cell culture ML Shuler (Eds) Plant Cell and Tissue Culture in Liquid Systems Carl Hanser Verlag Hanser 1–10
JW Seo CG Shin YE Choi (2003) ArticleTitleMass production of adventitious roots of Eleutherococcus sessiliflorus through the bioreactor culture J. Plant Biotechnol. 5 187–191
SH Son SM Choi SJ Hyung SR Yun MS Choi EM Shin YP Hong (1999) ArticleTitleInduction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng Biotechnol. Bioprocess Eng. 4 119–123 Occurrence Handle1:CAS:528:DC%2BD3cXktF2ku70%3D
W Wang ZY Zhang JJ Zhong (2005) ArticleTitleEnhancement of ginsenoside biosynthesis in high-density cultivation of Panax notoginseng cells by various strategies of methyl jasmonate elicitation Appl. Microbiol. Biol. 67 752–758 Occurrence Handle1:CAS:528:DC%2BD2MXltlCiur0%3D
PG Xiao (2002) The New Edition of the Annals of Traditional Chinese Medicine Chemical Industry Press Beijing
T Yoshikawa T Furuya (1987) ArticleTitleSaponin production by cultures of Panax ginseng transformed with Agrobacterium rhizonenes Plant Cell Rep. 6 449–453 Occurrence Handle1:CAS:528:DyaL1cXlt12jsA%3D%3D
KW Yu WY Gao EJ Hahn KY Paek (2002) ArticleTitleJasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer Biochem. Eng. J. 11 211–215 Occurrence Handle1:CAS:528:DC%2BD38XlvVequr8%3D
ZY Zhang JJ Zhong (2004) ArticleTitleScale-up of centrifugal impeller bioreactor for hyperproduction of ginseng saponin and polysaccharide by high-density cultivation of Panax notoginseng cells Biotechnol. Prog. 20 1076–1081 Occurrence Handle15296432 Occurrence Handle1:CAS:528:DC%2BD2cXjsFSnsbw%3D
GZ Zheng Z Liang (1978) ArticleTitleTissue culture of Panax notoginseng Acta Bot. Sin. 20 373–375
GZ Zheng SL Wang FY Gan (1989) ArticleTitleEstablishment of tissue culture from Panax notoginseng Chin. J. Biotechnol. 5 64–69
JJ Zhong QX Zhu (1995) ArticleTitleEffect of initial phosphate concentration on cell growth and ginsenoside saponin production by suspended cultures of Panax notoginseng Appl. Biochem. Biotechnol. 55 241–248 Occurrence Handle1:CAS:528:DyaK2MXhtVSktrzK Occurrence Handle10.1007/BF02786863
Author information
Authors and Affiliations
Corresponding author
Additional information
Revisions requested 13 July 2005; Revisions received 1 September 2005
Rights and permissions
About this article
Cite this article
Gao, X., Zhu, C., Jia, W. et al. Induction and Characterization of Adventitious Roots Directly from the Explants of Panax notoginseng . Biotechnol Lett 27, 1771–1775 (2005). https://doi.org/10.1007/s10529-005-3553-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-005-3553-4