Abstract
With the colony polymerase chain reaction (PCR), the specificity of newly prepared primer sets VCF2/VCR2, VCF3/VCR3, VCF4/VCR4, and VCF5/VCR5 to Ti or Ri plasmids in Agrobacterium strains were compared to that of the conventional set VCF/VCR. At first, control strains, which consisted of a nonpathogenic strain and phytopathogenic strains carrying Ti or Ri plasmid, were used. VCF3/VCR3 and VCF5/VCR5 were highly specific to all the phytopathogenic strains, whereas the others were not. These two primer sets were superior to VCF/VCR in their specificity with colony PCR to tumorigenic Agrobacterium strains isolated from apple saplings.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Suzaki, K., Yoshida, K. & Sawada, H. Detection of tumorigenic Agrobacterium strains from infected apple saplings by colony PCR with improved PCR primers. J Gen Plant Pathol 70, 342–347 (2004). https://doi.org/10.1007/s10327-004-0133-8
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10327-004-0133-8