Abstract
Purification and characterization of polyphenol oxidase (PPO) from Chinese parsley (Coriandrum sativum) were achieved. Crude PPO exhibited an enzyme activity of 1,952.24 EU/mL. PPO was partially purified up to 6.52x with a 10.89% yield using gel filtration chromatography. Maximal PPO activity was found at 35°C, pH 8.0 for 4-methylcatechol and at 40°C, pH 7.0 for catechol. PPO showed a higher affinity towards 4-methylcatechol, but a higher thermal stability when reacting with catechol. LCysteine was a better inhibitor than citric acid for reducing PPO activity at concentrations of 1 and 3mM in the presence of either substrate. Two 46 kDa isoenzymes were identified using SDS-PAGE. Isolation and characterization of Chinese parsley serves as a guideline for prediction of enzyme behavior leading to effective prevention of enzymatic browning during processing and storage, including inhibition and inactivation of PPO.
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Mathias ME. Magic, myth and medicine. Econ. Bot. 48: 3–7 (1994)
Dapkevicius A, Venskutonis R, van Beek TA, Linssen JPH. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J. Sci. Food Agr. 77: 140–146 (1998)
Park G, Kim HG, Kim YO, Park SH, Kim SY, Oh MS. Coriandrum sativum L. protects human keratinocytes from oxidative stress by regulating oxidative defense systems. Skin Pharmacol. Physiol. 25: 93–99 (2012)
Rajeshwari U, Andallu B. Medicinal benefits of coriander (Coriandrum sativum L.). Spatula DD. 1: 51–58 (2011)
Yoruk R, Marshall MR. Physiochemical properties and functions of plant polyphenol oxidase: A review. J. Food Biochem. 27: 361–422 (2003)
Mathew AG, Parpia HAB. Food browning as a polyphenol reaction. Vol. 19, pp. 75–132. In: Advances in Food Research. Chichester CO, Mrak EM, Stewart GF (eds). Academic Press, New York, NY, USA (1971)
Sapers GM, Hicks KB. Inhibition of enzymatic browning in fruits and vegetables. Vol. 405, pp. 29–43. In: Quality Factors of Fruits and Vegetables. Jen JJ (ed) American Chemical Society, Washington D.C., USA (1989)
Whitaker JR, Lee CY. Recent advances in chemistry of enzymatic browning. Vol. 600, pp. 2–7. In: Enzymatic Browning and Its Prevention. Lee CY, Whitaker JR (eds). American Chemical Society, Washington D.C., USA (1995)
Queiroz C, Mendes Lopes ML, Fialho E, Valente-Mesquita VL. Polyphenol oxidase: Characteristics and mechanisms of browning control. Food Rev. Int. 24: 361–375 (2008)
Nuñez-Delicado E, Serrano-Megías M, Pérez-López AJ, López-Nicolás JM. Polyphenol oxidase from Dominga table grape. J. Agr. Food Chem. 53: 6087–6093 (2005)
Ng AWR, Wong CW. Partial purification and characterization of polyphenol oxidase from round brinjal (Solanum melongena var. depressum). Int. Food Res. J. 22: 833–838 (2015)
Kumar VBA, Mohan TCK, Murugan K. Purification and kinetic characterization of polyphenol oxidase from Barbados cherry (Malpighia glabra L.). Food Chem. 110: 328–333 (2008)
Wong Y, Yu J. Laccase-catalyzed decolorization of synthetic dyes. Water Res. 33: 3512–3520 (1999)
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275 (1951)
Eidhin DN, Degn P, O’beirne D. Characterization of polyphenol oxidase from rooster potato (Solanum tuberosum CV Rooster). J. Food Biochem. 34: 13–30 (2010)
Ziyan E, Pekyardimci S. Purification and characterization of pear (Pyrus communis) polyphenol oxidase. Turk. J. Chem. 28: 547–557 (2004)
Dogan S, Turan Y, Ertruk H, Arslan O. Characterization and purification of polyphenol oxidase from artichoke (Cynaras colymus L.). J. Agr. Food Chem. 53: 776–785 (2005)
Gundoggmaz G, Doggan S, Arslan O. Some kinetic properties of polyphenol oxidase obtained from various Salvia species (Salvia viridis L., Salvia virgate Jacq. and Salvia tomentosa Miller). Food Sci. Technol. Int. 9: 309–315 (2003)
Dogru YZ, Erat M. Investigation of some kinetic properties of polyphenol oxidase from parsley (Petroselinum crispum, Apiaceae). Food Res. Int. 49: 411–415 (2012)
Dogru YZ, Erat M. Investigation of some kinetic properties of polyphenol oxidase from parsley (Petroselinum crispum, Apiaceae). Food Res. Int. 49: 411–415 (2012)
Cho YK, Ahn HK. Purification and characterization of polyphenol oxidase from potato: I. Purification and properties. J. Food Biochem. 23: 577–592 (1999)
Gauillard F, Richard-Forget F. Polyphenoloxidases from Williams pear (Pyrus communis L., cv Williams): Activation, purification and some properties. J. Sci. Food Agr. 74: 49–56 (1997)
Zhou P, Smith NL, Lee CY. Potential purification and some properties of Monroe apple peel polyphenol oxidase. J. Agr. Food Chem. 41: 532–536 (1993)
Yang CP, Fujita S, Ashrafuzzaman MD, Nakamura N, Hayashi N. Purification and characterization of polyphenol oxidase from banana (Musa sapientum L.) pulp. J. Agr. Food Chem. 48: 2732–2735 (2000)
Dincer B, Colak A, Aydin N, Kadioglu A, Güner S. Characterization of polyphenol oxidase from medlar fruits (Mespilus germanica L., Rosaceae). Food Chem. 77: 1–7 (2002)
Nagai T, Suzuki N. Partial purification of polyphenol oxidase from Chinese cabbage (Brassica rapa L.). J. Agr. Food Chem. 49: 3922–3926 (2001)
Oktay M, Küfreviolu I, Kocaçaliskan I, Sakirolu H. Polyphenol oxidase from Amasya apple. J. Food Sci. 60: 494–496 (1995)
Wesche-Ebleing P, Montgomery MW. Strawberry polyphenol oxidase: Extraction and partial characterization. J. Food Sci. 55: 1320–1325 (1990)
Zhou HW, Feng X. Polyphenol oxidase from Yali pear (Pyrus bretschneideri). J. Sci. Food Agr. 57: 307–313 (1991)
Altunkaya A, Gökmen V. Partial purification and characterization of polyphenoloxidase from durum wheat (Triticum durum L.). J. Cereal Sci. 55: 300–304 (2012)
Duangmal K, Apenten RKO. A comparative study of polyphenol oxidase from taro (Colocasia esculenta) and potato (Solanum tuberosum var. Romano). Food Chem. 64: 351–359 (1999)
Katwa LC, Ramakrishna M, Rao MRR. Purification and properties of polyphenol oxidase from mango peel (Mangifera indica var. Raspuri). J. Food Biochem. 6: 217–228 (1982)
Kahn V. Some biochemical properties of polyphenol oxidase from two avocado varieties differing in their browning rates. J. Food Sci. 42: 38–43 (1977)
Iyidogan NF, Bayindirli A. Effect of L-cysteine, kojic acid and 4-hexylresorcinal combination on inhibition of enzymatic browning in Amasya apple juice. J. Food Eng. 62: 299–304 (2004)
Gacche RN, Shete AM, Dhole NA, Ghole VS. Reversible inhibition of polyphenol oxidase from apple using l-cysteine. Indian J. Chem. Techn. 13: 459–463 (2006)
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Lin, H., Ng, A.W.R. & Wong, C.W. Partial purification and characterization of polyphenol oxidase from Chinese parsley (Coriandrum sativum). Food Sci Biotechnol 25 (Suppl 1), 91–96 (2016). https://doi.org/10.1007/s10068-016-0103-x
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DOI: https://doi.org/10.1007/s10068-016-0103-x