Abstract.
A novel pressure chamber system has been developed for the study of living cells under conditions of extremely high hydrostatic pressure up to 100 MPa (1 atm=0.101325 MPa). The temperature in the chamber is thermostatically controlled in the range from 2° to 80°C. Two high-pressure pumps are employed for continuous perfusion of the chamber with culture medium and a chemical solution under high hydrostatic pressure conditions. The chamber has a 2-mm-thick glass window 2 mm in diameter, with a minimum working distance of 3.8 mm. The chamber system is designed to be adaptable to a variety of microscopic and imaging techniques. Using this chamber system, we successfully carried out real-time observations of elongated Escherichia coli and rounded HeLa cells under pressure.
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Koyama, S., Miwa, T., Sato, T. et al. Optical chamber system designed for microscopic observation of living cells under extremely high hydrostatic pressure. Extremophiles 5, 409–415 (2001). https://doi.org/10.1007/s007920100213
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DOI: https://doi.org/10.1007/s007920100213