Summary.
Cerebrospinal fluid (CSF) carboxyterminally truncated amyloid-beta (Aβ) peptides, Aβ1-42 and tau protein were evaluated in 30 patients with frontotemporal lobe degenerations (FTLD), 30 Alzheimer’s disease (AD) patients and 30 non-demented disease controls (NDC) by Aβ-SDS-PAGE/immunoblot as well as commercial ELISAs for Aβ1-42 and total tau. FTLD displayed a significant drop of Aβ1-37 (p = 2.7 × 10−4), Aβ1-38 (p = 4.2 × 10−5) and Aβ1-42 (p = 3.3 × 10−4). Aβ1-42 was selectively decreased in AD (p = 8.5 × 10−10). Decreased Aβ1-38 enabled contrasts of beyond 85% to distinguish FTLD from AD and NDC patients, alone or in combination. Accordingly, low CSF Aβ1-37 and Aβ1-38 represent a biomarker candidate for FTLD and may reflect disease-specific changes of APP metabolism. Further validation should be carried out on dementias other than AD, diagnostically relevant control groups without dementia and without any evident affection of the central nervous system and subgroups of FTLD. Moreover, independent methods of measurement should be applied to CSF Aβ1-38.
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Abbreviations
- Aβ peptides:
-
Amyloid-beta peptides
- Aβ-SDS-PAGE/immunoblot:
-
amyloid-beta-sodium-dodecyl-sulphate-polyacrylamide-gel-electrophoresis with western immunoblot
- AD:
-
Alzheimer’s disease
- APP:
-
beta-amyloid precursor protein
- bicine:
-
N,N′-bis-[2-hydroxyethyl]glycine
- %C:
-
percentage of N,N′-bis-acrylamide
- CCD-camera:
-
charge coupled device camera
- CSF:
-
cerebrospinal fluid
- Ct-elongated:
-
carboxyterminally elongated
- Ct-truncated:
-
carboxyterminally truncated
- ECL:
-
enhanced chemiluminescence
- ELISA:
-
Enzyme Linked Immunosorbent Assay
- FTLD:
-
frontotemporal lobe degenerations
- MMSE:
-
Mini-Mental-Status Examination
- NDC:
-
non-demented disease controls
- NINCDS-ADRDA:
-
National Institute Neurological and Communicative Disorders and Stroke-Alzheimer’s Disease and Related Disorders Association
- SDS:
-
sodium dodecyl sulphate
- %T:
-
percentage of acrylamide
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InstitutionalAuthorNameWorld Medical Organisation (1996) ArticleTitleDeclaration of Helsinki Bri Med J 313 1448–1449
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Bibl, M., Mollenhauer, B., Wolf, S. et al. Reduced CSF carboxyterminally truncated Aβ peptides in frontotemporal lobe degenerations. J Neural Transm 114, 621–628 (2007). https://doi.org/10.1007/s00702-006-0618-z
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DOI: https://doi.org/10.1007/s00702-006-0618-z