Abstract.
A novel biosensor was fabricated by using a DNA-Ni(II)/MWNTs/Chitosan complex membrane, and the synergistic electrocatalytic effect of the complex membrane for the electro-oxidation of methanol was observed. The membrane composed of DNA, MWNTs and chitosan functioned as a support matrix for the immobilization of the electrocatalytic nickel cation. The good electrocatalytic activity of the resulting DNA-Ni(II)/MWNTs/chitosan/GC electrode was demonstrated by electro-oxidation of methanol in alkaline medium. A linear range from 0.2 to 5.0 mM for the detection of methanol in alkaline medium was observed with a rapid response (within 3 s) and a detection limit of 10 µM based on a signal-to-noise ratio of 3. In addition, the sensor exhibited good stability.
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Correspondence: Wanzhi Wei, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Hunan, Changsha 410082, P.R. China
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Liu, Y., Wei, W., Zhai, X. et al. A novel carbon nanotube-modified biosensor containing a dsDNA-Ni(II) complex membrane, and its use for electro-catalytic oxidation of methanol in alkaline medium. Microchim Acta 162, 245–251 (2008). https://doi.org/10.1007/s00604-007-0757-4
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DOI: https://doi.org/10.1007/s00604-007-0757-4