Abstract
Background
N6 methyladenosine (m6A) RNA methylation regulators play a key role in the occurrence and development of many tumors. However, the function of N6 methyladenosine (m6A) RNA methylation regulators in pancreatic adenocarcinoma (PAAD) has not been fully clarified.
Methods
We used data set from GEPIA 2, UALCAN, TIMER, TISIDB, CBioPortal database to analyze the gene expression of 20 major m6A RNA methylation regulators.
Results
Our study revealed that the irregularity of m6A regulators were associated with poor prognosis in PAAD. Meantime, 13 m6A regulators showed high expression in PAAD samples (ALKBH5, ELAVL1, FTO, HNRNPC, IGF2BP2, METTL14, METTL16 (METT10D), RBM15, VIRMA (KIAA1429), YTHDF1, YTHDF2, YTHDF3 and ZC3H13). In these regulators, we evaluated HNRNPC and IGF2BP2 were significantly correlated with worse outcomes and ALKBH5, IGF2BP2, METTL16 (METT10D), RBM15 were significantly correlated with PAAD in advanced stage. Moreover, we showed m6A regulators is correlated with Immuno-regulators' (Immunoinhibitors, Immunostimulators and MHC molecules) expression and levels of immune infiltration in PAAD. Bioinformatics further demonstrate m6A regulators were participated in revising in RNA processing.
Conclusions
Our study investigated that the m6A regulatory factors may serve as a biomarker and a potential target of immunotherapy for PAAD.
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Data availability
The data sets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- ACC:
-
Adrenocortical carcinoma
- BLCA:
-
Bladder urothelial carcinoma
- BRCA:
-
Breast invasive carcinoma
- CESC:
-
Cervical squamous cell carcinoma and endocervical adenocarcinoma
- COAD:
-
Colon adenocarcinoma
- ESCA:
-
Esophageal carcinoma
- GBM:
-
Glioblastoma multiforme
- HNSC:
-
Head and neck squamous cell carcinoma
- KIRC:
-
Kidney renal clear cell carcinoma
- LGG:
-
Brain lower grade glioma
- LIHC:
-
Liver hepatocellular carcinoma
- LUAD:
-
Lung adenocarcinoma
- LUSC:
-
Lung squamous cell carcinoma
- OV:
-
Ovarian serous cystadenocarcinoma
- PAAD:
-
Pancreatic adenocarcinoma
- PRAD:
-
Prostate adenocarcinoma
- STAD:
-
Stomach adenocarcinoma
- UCEC:
-
Uterine corpus endometrial carcinoma
- OS:
-
Overall survival
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Acknowledgements
This work was supported by the Yunnan Digestive Endoscopy Clinical Medical Center Foundation for Health Commission of Yunnan Province [2021LCZXXF-XH10]and[2022LCZXKF-XH09] of [ZX2019-01-02], Yunnan Fundamental Research Projects (grant NO. 202201AU070031), and we would like to express our sincere thanks to the database of GEPIA 2, UALCAN, TIMER, TISIDB, CBioPortal for supporting our research.
Funding
This study was founded by the Yunnan Digestive Endoscopy Clinical Medical Center Foundation for Health Commission of Yunnan Province [2021LCZXXF-XH10]and[2022LCZXKF-XH09] of [ZX2019-01-02], Yunnan Fundamental Research Projects (grant NO. 202201AU070031).
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YJ designed the study. TZ and PS performed the analyses and experiments. All authors wrote the manuscript, read and approved the manuscript.
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Zhang, T., Sheng, P. & Jiang, Y. m6A regulators are differently expressed and correlated with immune response of pancreatic adenocarcinoma. J Cancer Res Clin Oncol 149, 2805–2822 (2023). https://doi.org/10.1007/s00432-022-04150-7
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DOI: https://doi.org/10.1007/s00432-022-04150-7