Summary
Background
Polyunsaturated fatty acids play a key role in a number of biological functions. Rice bran oil (RBO) is rich in linoleic acid, an essential n–6 fatty acid. n–6 fatty acids are said to have proinflammatory effects as a result of an increase in n–6 fatty acidderived eicosanoids. RBO is also rich in γ–oryzanol, a compound from the unsaponifiable fraction, with antioxidant properties.
Objective
The aim of this work is to examine the effect of RBO—and/or γ–oryzanol—enriched diets on the regulation of the immune response.
Methods
4 week–old Balb/C mice were fed diets enriched with either RBO or high oleic–sunflower oil (HOSO), for one month. Serum samples, bone marrow–derived macrophages and lymphocytes from the spleen were collected.
Results
Compared to HOSO, our results show that RBO modulates the immune system by enhancing B–lymphocyte proliferation (6842 ± 2959 vs 10073 ± 4186 cpm; HOSO vs RBO; n = 10 per group) and TH1–type cytokines such as IL–2 (55.85 ± 18.2 vs 101.7 ± 21.6 pg/ml) or TNF–α (49.12 ± 18.6 vs 184.9 ± 46.2 pg/ml; HOSO vs RBO) in a significant way (n = 10 per group). Moreover, the reduction found in the TH2 cytokine IL–4 (7.59 ± 2.3 vs 4.48 ± 1.6 pg/ml) and IgE (56.9 ± 39.2 vs 42.4 ± 35.2 ng/ ml; HOSO vs RBO, n = 10 per group) levels suggests RBO may have antiallergenic properties. To elucidate the role of γ–oryzanol, a similar study was also carried out including diets enriched with refined RBO or HOSO containing γ–oryzanol (2 %). Our results suggest that although γ–oryzanol may modulate the immune system, it is not responsible for the overall immunostimulation effect seen for RBO.
Conclusions
RBO–enriched diets could be useful in situations where a potentiation of the immune response was required. The fatty acids composition, more than the unsaponifiable fraction, might be responsible for this effect.
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References
Paul WE (1999) The immune system: an introduction in fundamental immunology. Paul WE. 4th edition. Lippincott-Raven. Philadelphia
Chandra RK, Kumari S (1994) Nutrition and immunity: an overview. J Nutr 124:1433S–1435S
Kelley DS (2001) Modulation of the human immune and inflammatory responses by dietary fatty acids. Nutrition 17:669–673
Calder PC (1998) Fat chance of immunomodulation. Trends Immunol Today 19:244–247
Ray CC, Bouthillier L, Seidman E, Levy E (2004) New lipids in enteral feeding. Curr Opin Clin Nutr Metab Care 7:117–122
Calder PC (2003) N-3 Polyunsaturated fatty acids and inflammation: from molecular biology to the clinic. Lipids 38:343–352
Sugano M, Koba K, Tsuji E (1999) Health benefits of rice bran oil. Anticancer Res 19:3651–3657
Raghuram TC, Rukmini C (1995) Nutritional significance of rice bran oil. Indian J Med Res 102:241–244
Rukmini C, Raghuram TC (1991) Nutritional and biochemical aspects of the hypolipidemic action of rice bran oil: a review. J Am Coll Nutr 10:593–601
Rong N, Ausman LM, Nicolosi RJ (1997) Oryzanol decreases cholesterol absortion and aortic fatty streaks in hamsters. Lipids 32:303–309
Cicero AF, Gaddi A (2001) Rice bran oil and gamma-oryzanol in the treatment of hyperlipoproteinaemias and other conditions. Phytother Res 15:277–289
Jariwalla RJ (2001) Rice-bran products: phytonutrients with potential applications in preventive and clinical medicine. Drugs Exp Clin Res 27:17–26
Institute of Laboratory Animal Resource Commission of Life Sciences (1996) National Research Council. Guide for the care and use of laboratory animals. Washington (D/C). National Academy Press
Xaus J, Cardó M, Vellador AF, Soler C, Lloveras J, Celada A (1999) Interferon gamma induces the expression of p21waf and arrests macrophage cell cycle preventing induction of apoptosis. Immunity 2:103–113
Kelley DS, Warren JM, Simon VA, Bartolini G, Mackey BE, Erickson KL (2002) Similar effects of c9,t11-CLA and t10, c12-CLA on immune cells functions in mice. Lipids 37:725–728
Soler C, Garcia-Manteiga J, Valdes R, et al. (2001) Macrophages require different nucleoside transport systems for proliferation and activation. FASEB J 15:1979–1988
Xaus J, Comalada M, Valledor AF, Cardó M, Herrero C, Soler C, Llobreras J, Celada A (2001) Molecular mechanisms involved in macrophage survival, proliferation, activation or apoptosis. Immunobiol 204:543–550
Amati L, Cirimele D, Pugliese V, Covelli V, Resta F, Jirillo E (2003) Nutrition and immunity: laboratory and clinical aspects. Curr Pharm Des 9:1924–1931
Chandra S, Chandra RK (1996) Nutrition, immune response, and outcome. Prog Food Nutr Sci 10:1–65
Scavariello EM, Arellano DB (1998) Gamma-oryzanol: an important component in rice bran oil. Arch Latinoam Nutr 48:7–12
Nicolosi RJ, Ausman LM, Hegsted DM (1991) Rice bran oil lowers serum total and low density lipoprotein cholesterol and apo B levels in nonhuman primates. Atherosclerosis 88:132–142
Topping DL, Illman RJ, Roach PD, Trimble RP, Kambouris A, Nestel PJ (1990) Modulation of the hypolipidemic effect of fish oils by dietary fiber in rats: studies with rice and wheat bran. J Nutr 120:325–330
Jeffery NM, Yaqoob P, Newsholme EA, Calder PC (1996) The effects of olive oil upon rat serum lipid levels and lymphocyte functions appear to be due to oleic acid. Ann Nutr Met 40:71–80
Kuratko CN (2000) Proliferation of colonic lymphocytes in response to inflammatory cytokines is lower in mice fed fish oil than in mice fed corn oil. Cancer Lett 148:27–32
Hennig B, Meerarani P, Ramadass P, Watkins BA, Toborek M (2000) Fatty acid-mediated activation of vascular endothelial cells. Metabolism 49:1006–1013
Zusman I, Ben-Hur H, Budovsky A, et al. (2000) Transplacental effects of maternal feeding with high fat diets lipid exchange and response of the spleenic lymphoid system in mice offspring exposed to low doses of carcinogen. Int J Mol Med 6:337–343
Nomura I, Goleva E, Howell MD, Hamid QA, Ong PY, Hall CF, Darst MA, GAO B, Bouguniewicz H, Travers JB, Leung DYM (2003) Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J Immunol 171:3262–3269
Shishehbor MH, Hazen SL (2004) Inflammatory and oxidative markers in atherosclerosis. Curr Atheroscler Rep 6:243–250
Rasmussen LB, Kiens B, Pedersen BK, Richter EA (1994) Effect of diet and plasma fatty acids composition on immune status in elderly men. Am J Clin Nutr 59:572–577
Tauler P,Aguilo A, Cases N, et al. (2002) Acute phase immune response to exercise coexists with decreased neutrophil antioxidant enzyme defences. Free Radic Res 36:1101–1107
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S. Sierra and F. Lara-Villoslada contributed equally to this work.
This work was supported by Puleva Biotech SA funds. SA and FLV are recipients of a fellowship from the Fundación Universidad-Empresa, Universidad de Granada, Spain.
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Sierra, S., Lara–Villoslada, F., Olivares, M. et al. Increased immune response in mice consuming rice bran oil. Eur J Nutr 44, 509–516 (2005). https://doi.org/10.1007/s00394-005-0554-y
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DOI: https://doi.org/10.1007/s00394-005-0554-y