Abstract.
A new method is proposed for tracking fluorescing single molecules diffusing within a two-dimensional membrane. It is based on a confocal microscopy setup with a constantly rotating laser focus, which follows the position of the molecule. The optimization and efficiency of the method are theoretically studied for a broad range of experimentally realistic conditions. The proposed method allows for a long-time observation of diffusing molecules while allowing the application of fast spectroscopic techniques such as fluorescence decay time determination or fluorescence anisotropy measurements.
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Received: 14 January 2000 / Published online: 13 September 2000
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Enderlein, J. Tracking of fluorescent molecules diffusing within membranes. Appl Phys B 71, 773–777 (2000). https://doi.org/10.1007/s003400000409
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DOI: https://doi.org/10.1007/s003400000409