Abstract
A novel dynein-related transcript (designated DNEL1) from human adult testis has been identified that can encode a protein with a size of 91087 Da. The complete nucleotide sequence of the open reading frame is the first to be described for a human dynein-related gene. Northern blot analysis of mRNA from 16 different tissues has shown that DNEL1 is expressed specifically in testis. Analysis of somatic cell hybrids has mapped DNEL1 to Chromosome (Chr) 17. Analysis of a panel of 129 whole genome radiaton hybrid clones including 17q22-q25.3 has placed DNEL1 in 17q distal to the ERBA2L locus. DNEL1 shares a high degree of sequence identity and amino acid similarity with the C-terminal region of the outer arm axonemal dynein β-heavy chains derived from sea urchin and other species, but not to any gene encoding dynein intermediate or light chains described to date. The close similarity of DNEL1 to the C-terminal part of the axonemal β-heavy chain may suggest an origin from a common progenitor gene and the testis-specific pattern of expression a possible role in sperm development or motility.
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Affara, N.A., Bentley, E., Davey, P., Pelmear, A., Jones, M.H. (1994). Identification of novel gene sequences of the adult testis. Genomics, 22, 205–210.
Afzelius, B.A., Mossberg, B. (1989). Immotile-cilia syndrome (Primary Ciliary Dyskinesia including Kartagener syndrome. In The Metabolic and Molecular Bases of Inherited Disease, C.R. Scriver, A.L. Beaudet, W.S. Sly, D.M. Valle, eds. (New York: McGraw Hill Inc.), pp. 3943–3954.
Boehnke, M. (1992). Multipoint analysis for radiation hybrid mapping. Ann. Med. 24, 383–386.
Devereux, J., Haeberli, P., Smithies, O. (1984). A comprehensive set of sequence-analysis programs for WAX. Nucleic Acids Res. 12, 387–395.
Feinberg, A.P., Vogelstein, B. (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13.
Foster, J.W., Schafer, A.J., Critcher, R. Spillet, D.J., Feakes, R.W., Walter, M.A., Dominguez-Steglich, M., Guioli, S., Brook, DJ., Goodfellow, P.N. (1996). A high resolution whole genome radiation hybrid map of human chromosome 17q22-25.3, across the genes of GH and TK. Genomics 33, 185–192.
Furlong, R.A., Zhou, C.Y., Ferguson-Smith, M.A., Affara, N.A. (1996). Characterization of a kinesin related gene H-ATSV, within the tuberous sclerosis locus (TSC1) candidate region on chromosome 9q34. Genomics, in press.
Gepner, J., Hays, T.S. (1993). A fertility region on the Y chromosome of Drosophila melanogaster encodes a dynein microtubule motor. Proc. Natl. Acad. Sci. USA 90, 11132–11136.
Gibbons, I.R., Gibbons, B.H., Mocz, G., Assai, DJ. (1991). Multiple nucleotide-binding sites in the sequence of dynein microtubule motor. Nature 352, 640–643.
Handel, M.A., Kennedy, J.R. (1984). Situs inversus in homozygous mice without immotile cilia. J. Hered. 75, 498.
Hardy, R.W., Tokuyasu, K.T., Lindsley, D.L. (1981). Analysis of spermatogenesis in Drosophila melanogaster bearing deletions for Y-chromosome fertility genes. Chromosoma 83, 593–617.
Holzbaur, E.L.F., Mikami, A., Paschal, B.M., Vallee, R.B. (1994). Molecular characterization of cytoplasmic dynein. In Microtubules, J.S. Hyams, C.W. Lloyd, eds. (New York: Wiley-Liss, Inc.), pp. 251–267.
Koonce, M.P., Grissom, P.M., McIntosh, J.R. (1992). Dynein from Dictiostelium: primary structure comparisons between a cytoplasmic motor enzyme and flagellar dynein. J. Cell Biol. 119, 1597–1604.
Kozak, M. (1986). Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44, 283–292.
Kunkel, L.M., Smith, K.D., Bouyer, S.H., Borgaonkar, D.S., Wachtel, S.S., Miller, O.J., Breg, W.R., Jones, H.W., Rary, J.M. (1977). Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc. Natl. Acad. Sci. USA 74, 1245–1249.
Li, M.G., McGrail, M., Serr, M., Hays, T.S. (1994). Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte. J Cell Biol 126, 1475–1494.
Mitchell, D.R., Brown, K.S. (1994). Sequence analysis of Chlamidomonas alpha and beta dynein heavy chains. J. Cell Sci. 107, 635–644.
Narayan, D., Desai, T., Banks, A., Patanjali, S.R., Ravikumar, T.S., Ward, D.C. (1994). Localization of the human cytoplasmic dynein heavy chain (DNECL) to 14qter by fluorescence in situ hybridization. Genomics 22, 660–661.
Ogawa, K. (1991). Four ATP-binding sites in the midregion of the β-heavy chain of dynein. Nature 352, 643–645.
Pearson, W.R., Lipman, D.J. (1988). Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85, 2444–2448.
Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Hybridization of radiolabelled probes to immobilized nucleic acids. In Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press), pp. 9.47–9.55.
Schidlow, D.W., Katz, S.M. (1983). Immotile cilia syndrome. N. Eng. J. Med. 308, 595.
Staden, R. (1990). Finding protein coding regions in genomic sequences. Methods Enzymol. 183, 163–180.
Vaughan, K.T., Vallee, R.B. (1995). Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued. J. Cell Biol. 131, 1507–1516.
Walker, J.E., Saraste, M., Runswick, M.J., Gay, N.J. (1982). Distantly related sequences in the alpha-subunits and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1, 945–951.
Walter, M.A., Spillet, D.J., Thomas, P., Weissenbach, J., Goodfellow, P.N. (1994). A method for constructing radiation hybrid maps of the whole genome. Nature Genet. 7, 22–28.
Witman, G.B. (1989). Composition and molecular organization of the dyneins. In Cell Movement, F.D. Warner, P. Satir, I.R. Gibbons, eds., (New York: Alan R. Liss, Inc.), vol. 1, pp. 25–35.
Witman, G.B. (1992). Axonemal dyneins. Curr. Opin. Cell Biol. 4, 74–79.
Yoshida, T., Ioshii, S.O., Imanaka-Yoshida, K., Izutsu K. (1994). Association of cytoplasmic dynein with machete microtubules and spermatid nuclear envelope during spermiogenesis in rats. J. Cell Sci. 107, 625–633.
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Milisav, I., Jones, M.H. & Affara, N.A. Characterization of a novel human dynein-related gene that is specifically expressed in testis. Mammalian Genome 7, 667–672 (1996). https://doi.org/10.1007/s003359900202
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DOI: https://doi.org/10.1007/s003359900202