Abstract
The accumulation of different precursors of carotenoid biosynthesis in carotenoid-deficient mutants of Chlamydomonas reinhardtii was studied by HPLC-analysis. ζ-Carotene accumulated in several ac5 mutants, this character cosegregated with mutations in the ac5 gene. Two groups of ac5 mutants differing in ζ-carotene accumulation were distinguished. One (ac5–1) accumulated ζ-carotene in the dark but not in the light. The other (ac5–2) accumulated ζ-carotene under both dark and light conditions. ac5–2 strains accumulated more ζ-carotene in the dark than ac5-1 strains. Genetic data suggested that the mutations ac5–1 and ac5–2 were allelic. Pleiotropic effects of mutations in the ac5 gene included decreased levels of chlorophyll a and b and acetate requirement. The results are consistent with the presence of a defective ζ-carotene desaturase in ac5 mutants.
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Received: 27 October 1998 / Revision received: 1 February 1999 / Accepted: 16 February 1999
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Nikulina, K., Chunaev, A. & Boschetti, A. Accumulation of ζ-carotene in Chlamydomonas reinhardtii under control of the ac5 nuclear gene. Plant Cell Reports 19, 37–42 (1999). https://doi.org/10.1007/s002990050707
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DOI: https://doi.org/10.1007/s002990050707