Abstract
A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 μM 2,4-D and maintained on the same basal medium with 4.7 μM picloram+0.49 μM 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 μM picloram+13.3 μM BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.
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Received: 24 September 1998 / Revision received: 27 January 1999 / Accepted: 26 February 1999
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Myers, J., Simon, P. Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time. Plant Cell Reports 19, 32–36 (1999). https://doi.org/10.1007/s002990050706
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DOI: https://doi.org/10.1007/s002990050706