Abstract
The production of linolenic acid in mycelial lipids reached 0.31 mg/ml of culture broth when Mortierella isabellina was cultivated in a medium consisting of 2% octadecanol, 1% yeast extract, and 25 mmol/L of Mg2+ at 23°C for 5 days. Cultivation conditions were studied, and the results showed that (i) a suitable concentration of Mg2+ in the medium caused an increase in mycelial mass as well as linolenic acid production; (ii) when incubated at 23°C, maximal linolenic acid productivity was reached, although a higher content of the acid in total fatty acids was found at the lower temperature; (iii) the effect of substrate concentration on linolenic acid yield showed that the latter increased with concentration of substrate, and maximal linolenic acid yield was obtained with concentrations of 2% octadecanol and 1% yeast extract.
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Received: 27 November 2000 / Accepted: 22 June 2001
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Xian, M., Kang, Y., Yan, J. et al. Production of Linolenic Acid by Mortierella isabellina Grown on Octadecanol. Curr Microbiol 44, 141–144 (2002). https://doi.org/10.1007/s00284-001-0031-7
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DOI: https://doi.org/10.1007/s00284-001-0031-7