Abstract
Polyhydroxybutyrate (PHB) was produced by Ralstonia eutropha DSM 11348 (formerly Alicaligenes eutrophus) in media containing 20–30 g l−1 casein peptone or casamino acids as sole sources of nitrogen. In fermentations using media based on casein peptone, permanent growth up to a cell dry mass of 65 g l−1 was observed. PHB accumulated in cells up to 60%–80% of dry weight. The lowest yields were found in media without any trace elements or with casamino acids added only. The residual cell dry masses were limited to 10–15 g l−1 and did not contain PHB. The highest productivity amounted to 1.2 g PHB l−1 h−1. The mean molecular mass of the biopolymer was determined as 750 kDa. The proportion of polyhydroxyvalerate was less than 0.2% in PHB. The bioprocess was scaled up to a 300-l plant. During a fermentation time of 39 h the cells accumulated PHB to 78% w/w. The productivity was 0.98 g PHB l−1 h1.
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Received: 8 July 1998 / Accepted: 26 August 1998
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Bormann, E., Leißner, M., Roth, M. et al. Production of polyhydroxybutyrate by Ralstonia eutropha from protein hydrolysates. Appl Microbiol Biotechnol 50, 604–607 (1998). https://doi.org/10.1007/s002530051342
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DOI: https://doi.org/10.1007/s002530051342