Abstract.
Diastereoselective reduction of diketones with Lactobacillus kefir DSM 20587 was examined. The reduction of both oxo-functions proceeded highly diastereoselectively. (2R,5R)-Hexanediol 3 was produced starting from (2,5)-hexanedione 1 in quantitative yields with enantiomeric excess >99% and diastereomeric excess >99%. The reaction conditions were optimized: maximum yield of (2R,5R)-hexanediol was reached at pH 6, 30°C and with equal amounts of substrate and cosubstrate. The applicability of the system in fed-batch experiments was demonstrated. The feed specific biomass concentration required to reach maximal yield and selectivity in fed-batch mode was determined.
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Haberland, .J., Kriegesmann, .A., Wolfram, .E. et al. Diastereoselective synthesis of optically active (2R,5R)-hexanediol. Appl Microbiol Biotechnol 58, 595–599 (2002). https://doi.org/10.1007/s00253-002-0936-5
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DOI: https://doi.org/10.1007/s00253-002-0936-5