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Erratum to: Microb Ecol
DOI 10.1007/s00248-011-9943-3
The original version of this article unfortunately contained a mistake found under the Materials and Methods section for the PCR protocol under section Pyrosequencing of V5–V6 Hypervariable Regions”, what says:
“PCR amplifications were done as reported at the time [29] in a 25-μL reaction volume containing 2 μl (20 ng) DNA, 0.75 μM of each primer 807 F and 1050R designed by us (Table 1), 2.5 U Pfu Turbo® DNA polymerase (Stratagene, Inc., La Jolla, CA, USA), 1× Pfu reaction buffer, 0.6 mM dNTPs, 5% v/v dimethyl sulfoxide using the following PCR conditions: 2 min at 95°C, 30 cycles consisting of denaturation for 30 s at 95°C, a temperature touch down from 60 to 51°C (2°C every six cycles), 72°C for 1 min and a final extension of 72°C for 5 min.”
The correct wording should be:
“PCR amplifications were done as reported at the time [29] in a 25-μL reaction volume containing 2 μl (20 ng) DNA, 0.75 μM of each primer 807 F and 1050R designed by us (Table 1), 2.5 U Pfu Turbo® DNA polymerase (Stratagene, Inc., La Jolla, CA, USA), 1× Pfu reaction buffer, 0.6 mM dNTPs, 5% v/v dimethyl sulfoxide using the following PCR conditions: 2 min at 95°C, 30 cycles consisting of denaturation for 30 s at 95°C, annealing temperature starting at 60°C and reducing 0.2°C at every cycle, elongation at 72°C for 1 min and a final extension of 72°C for 5 min.”
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The online version of the original article can be found at http://dx.doi.org/10.1007/s00248-011-9943-3.
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Bohorquez, L.C., Delgado-Serrano, L., López, G. et al. Erratum to: In-depth Characterization via Complementing Culture-Independent Approaches of the Microbial Community in an Acidic Hot Spring of the Colombian Andes. Microb Ecol 63, 238 (2012). https://doi.org/10.1007/s00248-011-9977-6
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DOI: https://doi.org/10.1007/s00248-011-9977-6