Abstract.
Objective and design: In the present study, the effect of a synthetic peptide (H92–G102) identical to the C-terminus of murine S100A9 (mS100A9p) was investigated on adherent peritoneal cell function.
Materials and methods: For in vitro assays, peritoneal cells were obtained from the abdominal cavity of mice and incubated, with the different concentrations of mS100A9p, for 1 h, and then their spreading and phagocytosis activities were evaluated. For ex-vivo assays, cells obtained from animals treated for 1 h with the peptide were submitted to the mannose-receptor phagocytosis assay. Shorter homologue peptides to the C-terminus of mS100A9p were also evaluated on in vitro phagocytosis assays of Candida albicans particles.
Results: mS100A9p reduced both the spreading index and phagocytic activity, in vitro and ex-vivo, independent of the receptor evaluated. The homologue peptide corresponding to the H92–E97 region of mS100A9p, the zinc-binding motif, was responsible for such an effect.
Conclusion: These results suggest a modulator effect of the C-terminus of S100A9 protein on the function of adherent peritoneal cells.
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Received 4 November 2004; returned for revision 2 December 2004; accepted by I. Ahnfelt-Rønne 12 January 2005
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Pagano, R.L., Sampaio, S.C., Juliano, L. et al. The C-terminus of murine S100A9 inhibits spreading and phagocytic activity of adherent peritoneal cells. Inflamm. res. 54, 204–210 (2005). https://doi.org/10.1007/s00011-005-1344-y
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DOI: https://doi.org/10.1007/s00011-005-1344-y