Abstract
Somatic embryogenesis from leaf- and petiole-derived calli of Vitis rupestris was obtained with an efficiency of 3.2% and 4.2% of plated explants, respectively on two combinations of 6-benzyladenine and 2,4-dichlorophenoxyacetic acid (1/0.1 and 1/1 mgl−1) added to MS medium. Embryogenic callus, embryo subcultures and somatic embryogenesis from somatic embryos were obtained either in the presence of 1 mgl−1 indole-3-acetic acid or 0.1 mgl−1 indole-3-butyric acid added to MS or NN media. Within a 4-month culture, embryo germination occurred at a frequency of 13% of explanted embryos when chilling at 4°C was provided for two weeks and a combination of 6-benzyladenine (1 mgl−1) with indole-3-butyric acid (0.1 mgl−1) was added to NN medium supplemented with casein hydrolysate (250 mgl−1). A higher frequency (51%) was obtained in a longer culture time (9 months) when only indole-3-butyric acid was present in the medium and in absence of chilling.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog (1962)
- NN:
-
Nitsch and Nitsch (1969)
- NOA:
-
2-naphthoxyacetic acid
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Martinelli, L., Bragagna, P., Poletti, V. et al. Somatic embryogenesis from leaf- and petiole-derived callus of Vitis rupestris . Plant Cell Reports 12, 207–210 (1993). https://doi.org/10.1007/BF00237055
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DOI: https://doi.org/10.1007/BF00237055