Abstract
Serine proteinases secreted by adult Trichinella spiralis were isolated from excretory/secretory products (ES) of in-vitro-cultured parasites by affinity chromatography with p-benzamidine-celite. The purified enzymes had molecular weights of approximately 18, 40, and 50 kDa and displayed enzyme activity against a range of low-molecular-weight substrates, gelatin, and azocasein. The antigenicity of these parasite proteinases was demonstrated by the inhibition of enzymatic activity with IgG purified from infected hosts. The inactivation of major secreted proteinases of adult T. spiralis by immune antibody could presumably contribute to impairment of the survival of the parasite in sensitized hosts.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 29 January 2000 / Accepted: 8 February 2000
Rights and permissions
About this article
Cite this article
Todorova, V., Stoyanov, D. Partial characterization of serine proteinases secreted by adult Trichinella spiralis . Parasitol Res 86, 684–687 (2000). https://doi.org/10.1007/PL00008552
Issue Date:
DOI: https://doi.org/10.1007/PL00008552