Abstract
Objective and Design: Ultraviolet (UV) exposure induces local immunosuppression and inflammation in human skin. Cytokines are, in part, responsible for these responses. To investigate the effects of UV-induced gene expression at the molecular level we established a sensitive in vivo/ex vivo method for a comparative quantification of cytokines and receptors involved in the local skin immune reactions.¶Material and Methods: Specific mRNA levels of human UV-irradiated skin were determined by real time quantification (TaqManTM RT-PCR). Highly efficient PCR-reaction conditions were obtained by designing very short PCR-templates (72-87bp). The most sensitive PCR-conditions were obtained by optimisation of primer and Mn(OAc)2-concentrations, which led to significant PCR signals (CT-value) of less than 36 cycles. A strong correlation between PCR efficiency of the internal control (GAPDH) compared to targets (IL-1β, IL-10, IL-10r, TNFα, IL-7) allowed the use of δδCT-method to quantify comparable mRNA levels.¶Results: Interleukin-1β (IL-1β), Interleukin-10 (IL-10), and tumour necrosis factor alpha (TNFα) mRNA levels were increased in a time- and dose-dependent manner. Interleukin-1β induction reached a maximum (approx. 44-fold) 6 h after a UV-dose equivalent to 3 times the minimal erythemal doses just perceptible (MEDjp). Maximal TNFα mRNA expression (approx. 14-fold) was also detected 6 h after UV exposure. Interleukin-10 mRNA induction reached a maximum of approximately 14-fold 24 h after UV-irradiation of 3 MEDjp. Time- and dose-dependent changes in Interleukin-7 and Interleukin-10 receptor mRNA levels did not occur after UV-irradiation.¶Conclusions: Time-distinct gene induction of IL-1β, TNFα and IL-10 is involved in UV-induced immune reactions, but no considerable changes were found for IL-10r or IL-7.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received 9 November 1999; returned for revision 10 January 2000; accepted by I. Ahnfelt-Rønne 27 January 2000
Rights and permissions
About this article
Cite this article
Brink, N., Szamel, M., Young, A. et al. Comparative quantification of IL-1β, IL-10, IL-10r, TNFα and IL-7 mRNA levels in UV-irradiated human skin in vivo. Inflamm. res. 49, 290–296 (2000). https://doi.org/10.1007/PL00000209
Published:
Issue Date:
DOI: https://doi.org/10.1007/PL00000209