Abstract
The absorption, fluorescence, fluorescence polarization and circular dichroism spectra of an artificial complex human serum albumin-chlorophyll-a were measured at 20°C in buffer solution (pH 7.2) and the effect of detergents (digitonin, sodium lauryl sulfate, above critical micelle concentration) was studied. A strong chlorophyll-chlorophyll interaction found in the complex is removed by detergent treatment. The ionic detergent sodium lauryl sulphate induces pheophytinization and conformational changes in the protein part of the complex leading to changes in the absolute configuration of the transition moments of the pigment. Profound changes in the fluorescence polarization spectra are caused by both detergents. The effects of detergents suggest that the possibility of similar phenomena in treating plant material should be rigorously considered.
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Dedicated to Prof. I. Tarján on his 70th birthday.
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Szalay, L., Tombácz, E., Várkonyi, Z. et al. Detergent effects on an albumin-chlorophyll complex model of photosynthetic protein-pigment complexes. Acta Physica 53, 225–235 (1982). https://doi.org/10.1007/BF03156192
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DOI: https://doi.org/10.1007/BF03156192