Abstract
Verticillium dahliae is a strong soil-borne pathogen that causes early dying of potato plants. Pathogen population dynamics were studied during disease development following combined inoculations of potato withV. dahliae and various otherVerticillium species. In greenhouse and field studies, threeVerticillium species were examined:V dahliae, V. albo-atrum ‘group 2’ andV tricorpus. Potato plants were inoculated with two out of the three species in various combinations of an aggressive (V. dahliae) and weak (V albo-atrum ‘group 2’ orV tricorpus) pathogen either on the same date or with a weak species followed by an aggressive species four days later. Plant and soil samples were collected and relative population levels (RPLs) of each pathogen were determined using polymerase chain reaction (PCR) techniques. In combinations where pathogens were inoculated at the same time, RPLs of the weaker species did not exceed those of the aggressive species. In combinations where the weaker species were inoculated first, followed byV. dahliae four days later, the two weaker species were still not able to exceed RPLs ofV. dahliae. visual wilt symptoms, however, were significantly lower than co-inoculations at the same time and the single inoculation studies. Implications of these findings on epidemiological aspects of these hostpathogen interactions are discussed.
Resumen
Verticillium dahliae es un poderoso patógeno habitante del suelo que causa muerte prematura de plantas de papa. La dinámica poblacional del patógeno ha sido estudiada durante el desarrollo de la enfermedad después de inoculaciones combinadas de papa conV. dahliae y varias otras especies deVerticillium. En estudios de invernadero y campo, se examinaron tres especies deVerticillium: V. dahliae, V. albo-atrum ‘grupo 2’yV. tricorpus. Plantas de papa fueron inoculadas con dos de las tres especies en diferentes combinaciones de un patógeno agresivo (V dahliae) y uno debil (V albo-atrum ‘grupo 2’ oV. tricorpus) en la misma fecha o con una especie débil seguida de una agresiva cuatro días después. Se colectaron muestras de planta y de suelo y se determinaron los niveles de poblacion relativa (RPLs) utilizando técnicas de reacción en cadena de la polimerasa (PCR). En las combinaciones donde los patógenos fueron inoculados al mismo tiempo, los RPLs de las especies débiles no excedieron a aquellas de las especies agresivas. En combinaciones en las que las especies débiles fueron inoculadas primero, seguidas deV. dahliae cuatro dias después, las especies más débiles no fueron capaces de exceder los RPLs deV. dahliae. Los síntomas visuales, sin embargo fueron significativamente menores que las co-inoculaciones simples de un mismo tiempo. Se discuten las implicancias de estos hallazgos sobre aspectos epidemiológicos de las interacciones huésped-patógeno.
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Abbreviations
- VD:
-
Verticillium dahliae
- VT:
-
Verticillium tricorpus
- VA2:
-
Verticillium albo-atrum
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RPL, relative population level Contribution: AAFC-CLRC # 1057
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Robinson, N., Piatt, H.W. & Hale, L.R. Verticillium dahliae interactions with V. albo-atrum and V. tricorpus and their effects on verticillium wilt disease development in potato. Amer J of Potato Res 84, 229–235 (2007). https://doi.org/10.1007/BF02986272
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DOI: https://doi.org/10.1007/BF02986272