Abstract
A continuous biparticle fluidized-bed reactor is developed for the simultaneous fermentation and purification of lactic acid. In this processing scheme, bacteria are immobilized in gelatin beads and are fluidized in a columnar reactor. Solid particles with sorbent capacity for the product are introduced at the top of the reactor, and fall counter currently to the biocatalyst, effectingin situ removal of the inhibitory product, while also controlling reactor pH at optimal levels. Initial long-term fermentation trials using immobilizedLactobacillus delbreuckii have demonstrated a 12-fold increase in volumetric productivity during absorbent addition as opposed to control fermentations in the same reactor. Unoptimized regeneration of the loaded sorbent has effected at least an eightfold concentration of lactic acid and a 68-fold enhancement in separation from glucose compared to original levels in the fermentation broth. The benefits of this reactor system as opposed to conventional batch fermentation are discussed in terms of productivity and process economics.
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References
Atkinson, B. and Mavituna, F. (1991),Biochemical Engineering and Biotechnology Handbook. 2 ed. Stockton, New York.
Lipinsky, E. S. and Sinclair, R. G. (1986),Chem. Eng. Prog. 82, 26–32.
Chemical Marketing Reporter. March 14, 1994, pp. 28–36.
Millis, J. (1993)Amer. Chem. Soc. 38, 297–299.
Ohleyer, E., Blanch, H. W., and Wilke, C. R. (1985)Appl. Biochem. Biotechnol. 11, 317–331.
Evangelista, R. L., Mangold, A. J., and Nikolov, Z. L. (1994)Appl. Biochem. Biotechnol. 45, 131–144.
Mercier, P., Yerushalmi, L., Rouleau, D., and Dochain, D. (1992)J. Chem. Technol. Biotechnol. 55, 111–121.
Buchta, K. (1983),Lactic Acid, inBiotechnology: A Comprehensive Treatise Biomass, Microorganisms for Special Applications Microbial Products I, Energy from Renewable Resources, Dellweg, H., ed., Verlag Chemie, Weinheim.
Kirk-Othmer, X. (1981),Encyclopedia of Chemical Technology, 3 ed., vol. 13, Grayson, M., ed., John Wiley: New York.
King, C. J. (1992),Chem Tech 22(5), 285–291.
Yeh, P. L. H., Bajpai, R. K., and Iannotti, E. L. (1991),J. Fermentation Bioeng. 71(1), 75–77.
Kuhn, R., Peretti, S., and Ollis, D. (1993)Appl. Biochem. Biotechnol. 39/40, 401–413.
Melzoch, K. and Konopaskova, L. (1993)Biotechnol. Lett. 15(5), 517–520.
Andrews, G. F. and Fonta, J. P. (1989),Appl. Biochem. Biotechnol. 20, 375–390.
Stenroos, S. L., Linko, Y. Y., and Linko, P. (1982),Biotechnol. Lett. 4(3), 159–164.
Guoquiang, D., Kaul, R., and Mattiasson, B. (1991),Appl. Microbiol. Biotechnol. 36, 309–314.
Hang, Y. D., Hamamci, H., and Woodams, E. E. (1989),Biotechnol. Lett. 11(2), 119–120.
Yabannavar, V. M. and Wang, D. I. C. (1991),Biotechnol. Bioeng. 37, 1095–1100.
Vick Roy, T. B., Blanch, H. W., and Wilke, C. R. (1982),Biotechnol. Lett. 4 (8), 483–488.
Shi, Z., Shimizu, K., Iijima, S., Morisue, T., and Kobayashi, T. (1990),J. Fementation Bioeng. 70(6), 415–419.
Kulozik, U., Hammelehle, B., Pfeifer, J., and Kessler, H. G. (1992),J. Biotechnol. 22, 107–116.
Venkatesh, K. V., Okos, M. R., and Wankat, P. C. (1993),Process Biochem. 28, 231–241.
Seevaratnam, S., Holst, J. O., Hjorleifsdottir, S., and Mattiasson, B. (1991),Bioprocess Eng. 6, 35–41.
Martin, M. S., Pazos, C., and Coca, J. (1992),J. Chem. Tech. Biotechnol. 54, 1–6.
Garcia, A. A. (1991),Biotechnol. Prog. 7, 33–42.
Galliot, F. P., Gleason, C., Wilson, J. J., and Zwarick, J. (1990)Biotechnol. Prog. 6, 370–375.
Srivastava, A., Roychoudhury, P. K., and Sahai, V. (1992),Biotechnol. Bioeng. 39, 607–613.
Davison, B. H. and Scott, C. D. (1992),Biotechnol. Bioeng. 39, 365–368.
Davison, B. H. and Thompson, J. E. (1992),Appl. Biochem. Biotechnol. 34, 431–439.
Godia, F., Casas, C., and Sola, C. (1987),Process Biochem. 22, 43–48.
Davison, B. H. and Scott, C. D. (1988),Appl. Biochem. Biotechnol. 18, 19–34.
Scott, C. D. (1987),Ann. NY Acad. Sci. 501, 487–493.
Kaufman, E. N., Cooper, S. P., and Davison, B. H. (1994),Appl. Biochem. Biotechnol. 45, 545–554.
Nakagawa, M., Nakamura, I., and Kobayashi, T. (1975),J. Ferment. Technol. 53(3), 127–134.
Fluor Daniel Inc. (1994),Economic Assessment of Ethanol Production Comparing Traditional and Fluidized Bed Bioreactors. Oak Ridge National Laboratory, DOE P.O. No. 32X-SM954V.
Rohm and Haas Company (1975),Amberlite IRA-35.
Chem Systems Inc. (1984),Technical and Economic Assessment of Processes for the Production of Butanol and Acetone. The Jet Propulsion Laboratory.
Chem Systems Inc. (1986),Implications of ORNL Immobilization Technology on Citric Acid Production. Jet Propulsion Laboratory.
Hanson, T. P. and Tsao, G. T. (1972)Biotechnol. Bioeng. 14, 233–252.
Guoqiang, D., Kaul, R., and Mattiasson, B. (1992),Appl. Microbiol. Biotechnol. 37, 305–310.
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The submitted manuscript has been authored by a contractor of the US government under contract No. DE-AC05-84OR21400. Accordingly, the US government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for US government purposes.
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Kaufman, E.N., Cooper, S.P., Clement, S.L. et al. Use of a biparticle fluidized-bed bioreactor for the continuous and simultaneous fermentation and purification of lactic acid. Appl Biochem Biotechnol 51, 605–620 (1995). https://doi.org/10.1007/BF02933462
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DOI: https://doi.org/10.1007/BF02933462