Abstract
The impact ofIxodes ricinus salivary gland extract (SGE) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen,B. afzelii, was determined using flow cytometry. SGE injected together with spirochetes reduced the numbers of leukocytes and γδ-T lymphocytes in infected epidermis at early time-points post infection. In draining lymph nodes, the anti-inflammatory effect of SGE was manifested by the decrease of total cell count compared with that in mice treated with inactivated SGE. Changes in subpopulations of immunocompetent cells apparently reflected the effect of SGE on the proliferation of spirochetes in the host. The significance of tick saliva anti-inflammatory effect for saliva activated transmission ofB. afzelii is shown.
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Abbreviations
- BP:
-
band pass
- FACS:
-
fluorescein-activated cell sorter
- FCS:
-
fetal calf serum
- FITC:
-
fluorescein isothiocyanate
- PBS:
-
phosphate-buffered saline
- p.i.:
-
post infection
- PMNs:
-
polymorphonuclear cells
- RPE:
-
rhodamine-phycoerythrin
- SAT:
-
saliva-activated transmission
- SGE:
-
salivary gland extract
- TCR:
-
T-cell receptor
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This work was supported by grant of theGrant Agency of the Czech Republic (no. 524/02/0901) and by grant of theMinistry of Education, Youth and Sports of the Czech Republic (no. MSM 123100003). This study is a part of the research project of theInstitute of Parasitology, Academy of Sciences of the Czech Republic (Z6 022 909). Experiments comply with the current laws of the Czech Republic.
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Severinová, J., Salát, J., Kročová, Z. et al. Co-inoculation ofBorrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice. Folia Microbiol 50, 457–463 (2005). https://doi.org/10.1007/BF02931430
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DOI: https://doi.org/10.1007/BF02931430